there has been a renewed interest in edible films made of renewable and natural polymers such as protein, polysaccharide and lipids. natural polymers derived from natural sources like food protein, offer the greatest opportunities because of their biodegradability and their ability to supplement nutritional value of foods. faba bean is a valuable source of protein and is cultivated in large qua...

some biochemical properties of silver carp surimi prepared by application of acid-alkali aided methods were investigated and compared to that of derived by conventional method. in terms of total protein solubility and recovery, lipid reduction, and total pigment extractability and myoglobin removal there was a significant (p

Solubility of proteins on overexpression in Escherichia coli is a manifestation of the net effect of several sequence-dependent and sequence-independent factors. This study aims to delineate the relationship between the primary structure and solubility on overexpression. The amino acid sequences of proteins reported to be soluble or to form inclusion bodies on overexpression in E. coli under no...

I n the effort to understand living cells, knowledge about molecular details competes with knowledge of collective properties of large systems of molecules. On the one hand, molecular structures and interactions strongly suggest how the cell’s molecular machines work and give rise to rich metabolic diagrams. On the other hand, subtle collective properties such as the entropy and free energy rep...

introduction : soybean is an excellent plant protein source with diverse applications in food systems. despite numerous commercial applications and rich nutritional properties of soybeans, soy proteins are sensitive to heat and other damaging agents during food processing which can limit their applications in food industries. maillard reaction includes a series of chemical reactions between the...

A reproducible method for comparing the effects of treatment with milk products on the acid solubility of enamel surfaces is described. Using this method it was found that treatment with cows’ milk reduced solubility by more than 20 per cent, regardless of whether it was raw or pasteurized whole or skim milk. Reconstituted whole and reconstituted skim milk gave similar reductions. Similar depre...

Background: The increase of the protein expression via ribosomal manipulation is one of the suggested cellular mechanisms involved in EnBase fed-batch mode of cultivation. However, this system has not been implemented for cytotoxic proteins.Objectives: Here, the expression pattern of α-Luffin, a ribosome inactivation protein (RIP) with an innate toxicity,...

Sufficient solubility of the active protein in aqueous solution is a prerequisite for crystallization and other structural studies of proteins. In this study, we have developed a simple and effective in vivo screening system to select for functionally active proteins with increased solubility by using Plasmodium falciparum dihydrofolate reductase (pfDHFR), a well-known malarial drug target, as ...

Accumulation of insoluble protein in cells is associated with aging and aging-related diseases; however, the roles of insoluble protein in these processes are uncertain. The nature and impact of changes to protein solubility during normal aging are less well understood. Using quantitative mass spectrometry, we identify 480 proteins that become insoluble during postmitotic aging in Saccharomyces...