Introduction: Antibiotic supplements are regularly used in neuronal culture media to control contamination however, they can interfere with the neuronal excitability and affect electrophysiological properties. Therefore, in this study, the effect of penicillin/streptomycin supplements on the spontaneous electrophysiological activity of hippocampal pyramidal neurons was examined. Methods: Electr...

OBJECTIVE To evaluate the effects of Chinese herbal medicine for warming kidney on proliferation of normal human mammary epithelial cells in primary culture. METHODS The normal human mammary epithelial cells were dissociated by digestion with collagenase type I. The morphological identification of normal human mammary epithelial cells in primary culture was determined under inverted phase con...

Herein, we presented the first report on bluetongue (BT) disease in 10 pregnant camels in a herd fromKerman province, Iran. All sera samples were tested serologically (AGID, C-ELISA). We also used the Razi-BK cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ECE) to culture and isolate the BT virus. Efforts to culture and isolation of...

BACKGROUND There is little guidance available to healthcare practitioners about what tools they might use to assess the patient safety culture. OBJECTIVE To identify useful tools for assessing patient safety culture in primary care organizations in Europe; to identify those aspects of performance that should be assessed when investigating the relationship between safety culture and performanc...

Genus Leishmania is a protozoan parasite that causes different severe diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, for mass cultivation and the most expensive ingredient in these media. In the present work, the efficacy of chicken serum was evaluated in Leishmania culture media. The results indicated that, the (10%) chicken serum enriched culture medium su...

Rickettsia rickettsi was isolated and propagated in primary cell cultures derived from experimentally infected guinea pigs. Organisms were recognized as early as 3 days after cultures were initiated.

introduction: an induction of osteogenic differentiation in rat bone marrow stromal cell cultures by dexamethasone was reported. materials and methods: the marrow stromal cells obtained from 4 to 6 weeks - old spruge-dawely male rats were grown in primary culture for 7 days and then subcultured for 20 days. the cells were cultured in either dmem medium containing 15% fetal calf serum, antibioti...