A real-time multiplex PCR procedure with melting curve analysis, using the green fluorescence dye SYBR Green I, was developed for rapid and reliable identification of Plum pox virus (PPV) isolates of strains D and M. Members of the different strains were identified by their distinctive melting temperatures (T(m)s); 84.3-84.43 degrees C for D isolates, and 85.34-86.11 degrees C for M isolates. T...

RNA silencing is a powerful technology for molecular characterization of gene functions in plants. A commonly used approach to the induction of RNA silencing is through genetic transformation. A potent alternative is to use a modified viral vector for virus-induced gene silencing (VIGS) to degrade RNA molecules sharing similar nucleotide sequence. Unfortunately, genomic studies in many allogamo...

Plum pox virus (PPV), sert çekirdekli meyvelerde önemli verim kayıplarına sebep olan Şarka hastalığının etmenidir. PPV, Türkiye’nin farklı bölgelerinde sınırlı olarak tespit edilmesine rağmen, bugüne kadar Burdur iline ait herhangi bir kayıt bulunmamaktadır. Bu çalışmada, 2016-2019 yılları arasında ilinde PPV’nin varlığının belirlenmesine yönelik serolojik ve moleküler yöntemler kullanılarak ya...

RNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apricot is performed. RNA-Seq has been applied to analyse the gene expression changes induced by PPV ...

The sequence of 1232 nucleotides from the 3' region of the plum pox virus (PPV) RNA has been determined. This sequence contains one long open reading frame (ORF). The sequence of the 17 amino-terminal amino acids of the PPV capsid protein was determined chemically. An identical amino acid sequence was found in the translation product of the large ORF, starting at amino acid 330, which is alanin...

Understanding biological mechanisms that regulate emergence of viral diseases, in particular those events engaging cross-species pathogens spillover, is becoming increasingly important virology. Species barrier jumping has been extensively studied animal viruses, and the critical role a suitable intermediate host viruses-generated human pandemics highly topical. However, studies on involving pl...

In order to sustain productivity, growers are implementing fruit trees provide plum pox virus (PPV) resistant varieties. Unfortunately, classical breeding approaches have failed develop RNA interference (RNAi) silencing, as an alternate strategy, has been shown be efficient approach combat PPV disease (sharka). resistance based on RNAi demonstrated in natural conditions for over 10 years. Exper...

A DNA biosensor for detection of specific oligonucleotides sequences of Plum Pox Virus (PPV) in plant extracts and buffer is proposed. The working principles of a genosensor are based on the ion-channel mechanism. The NH2-ssDNA probe was deposited onto a glassy carbon electrode surface to form an amide bond between the carboxyl group of oxidized electrode surface and amino group from ssDNA prob...

The nucleotide sequence of the 3'-terminal 4773 nucleotides of the RNA of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from Egypt (PPV-El Amar) was determined. The sequenced region covers the carboxy terminus of the cylindrical inclusion (CI) gene, and the putative 6K protein, the NIa protease, the NIb RNA polymerase and the coat protein genes, linked together a...