Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...

Background. Assisted reproductive technology has been linked to the increased incidence of monozygotic twinning. It is of clinical importance due to the increased risk of complications in multiple pregnancies in general and in monozygotic twins in particular. Case. A 29-year-old female, nulligravida underwent her first IVF cycle. Three poor-quality cleavage stage embryos were transferred result...

Background To compare the pregnancy outcomes after two embryos versus three embryos transfers (ETs) in women undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles. MaterialsAndMethods This retrospective study was performed on three hundred eighty seven women with primary infertility and with at least one fresh embryo in good quality in order to transfer at each ...

STUDY QUESTION Is the quality of the human embryos generated by twinning in vitro comparable to the quality of the embryos created by fertilization? SUMMARY ANSWER Our data suggest that the human twin embryos created in vitro are unsuitable not only for clinical use but also for research purposes. WHAT IS KNOWN ALREADY Pregnancy from in vitro generated monozygotic twins by embryo splitting ...

PURPOSE The aim of this study was to explore factors contribute to the success of PGD cycles for monogenic diseases. METHODS During a 3-year period (January 2009 to December 2012), 184 consecutive ICSI-PGD cycles for monogenic diseases reaching the ovum pick-up and fresh embryo-transfer stage performed at the Reproductive Medicine Center of The First Affiliated Hospital Of Sun Yat-sen Univers...

OBJECTIVE To compare the laboratory outcomes of intracytoplasmic sperm injection (ICSI) and conventional insemination using sibling oocytes in poor prognosis IVF cycles where ICSI is not indicated. METHODS Couples undergoing IVF with following conditions were enrolled: history of more than 3 years of unexplained infertility, history of ≥3 failed intrauterine insemination, leukocytospermia or ...

background: determination of oocyte and embryo quality are one of the most important goals in ivf. anti-mullerian hormone (amh) is secreted by the ovarian granulosa cells into blood flow and follicular fluid. follicular fluid anti-mullerian hormone level is probably a marker of activity of granulose cells. objective: to evaluate whether high level of follicular fluid anti-mullerian hormone leve...

Background: The biochemical characteristics of the follicular fluid (FF) play essential role in oocyte quality, the subsequent fertilization potential and embryo development. The oocyte quality and metabolism largely depends on follicular microenvironment. Present study aimed to investigate the relationship between oocyte quality and FF homocysteine (Hcy) level in women undergoing to IVF treatm...