Pedigree information and phenotypic records are the key features in animal genetics and breeding. Otherwise best methods for genetic evaluation and estimation of parameters will be tending to no correct results. This study was aimed to investigate and verify pedigree relations among 76 individuals who were genotyped for 3 fluorescent labeled microsatellite markers picked up from FAO-ISAG joint ...

Thirteen microsatellite loci were isolated from the coqui frog (Eleutherodactylus coqui) and optimized for future research. The loci were screened across 37 individuals from two Puerto Rican populations. Loci were variable with the number of alleles per locus ranging from three to 38. Polymorphic information content ranged from 0.453 to 0.963 and observed heterozygosity for each population rang...

DNA fingerprinting has become an important tool for diversity assessment and varietal identification in plant breeding programs. Semi- random PCR primers targeting intron-exon splice junctions (ISJ) were used to evaluate the potential of these markers in identification and classification of rice genotypes. A total of 12 ISJ primers were used for screening fourteen Egyptian rice genotypes, inclu...

Polymorphic malcode remains one of the most troubling threats for information security and intrusion defense systems. The ability for malcode to be automatically transformed into to a semantically equivalent variant frustrates attempts to construct a single, simple, easily verifiable representation. We present a quantitative analysis of the strengths and limitations of shellcode polymorphism an...

PREMISE OF THE STUDY Microsatellite primers were developed for Paeonia delavayi and P. ludlowii (Paeoniaceae) to study their population genetics and phytogeography. METHODS AND RESULTS Nine polymorphic nuclear microsatellite loci were isolated from an enriched library of P. delavayi and primers were designed. The number of alleles per locus ranged from two to 16; the observed and expected het...

A polymorphic tetranucleotide (GAAT)n microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)5, (GAAT)6, (GAAT)7 and (GAAT)8, including 9 of...

The blue fox, belonging to the family Canidae, is a coat color variant of the native arctic fox (Alopex lagopus). To date, microsatellite loci in blue fox are typically amplified using canine simple sequence repeat primers. In the present study, we constructed an (AC)n enrichment library, and isolated and identified 17 polymorphic microsatellite markers for blue fox. The number of alleles per l...

the present study was to construct a parentage verification system for iranian caspian horse. a total number of 45 caspian horse samples including 14 foals for parentage verification, 17 stallion and 14 mare for individual identification were genotyped. genomic dna was extracted from whole blood and the genotype were analysed by pcr procedure and using 7 microsatellite markers (aht04, hms03, hm...