results of 50 patients, 20 were men and 30 women with mean age of 61.1 ± 16.9 years ranged between 22 and 85 years. all samples of pterygia had positive results for adenoviruses dna with polymerase chain reaction, but none of the negative control groups displayed adenoviruses. the pterygium group and the control groups were β-globin positive. direct sequencing of pcr products confirmed adenovir...

background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...

conclusions: vegetables are a new source of h. pylori and accurate washing of vegetables improves such contaminations. results: the results showed that 44 of 460 samples (9.56%) were positive for h. pylori using the culture method. the polymerase chain reaction technique showed that 50 of 460 samples (10.86%) were positive for h. pylori. un-washed leek, traditional salad, un-washed basil and un...

abstract background brucellosis is endemic in iran, and is seen in all age groups, including children. its diagnosis in childhood needs a high index of suspicion. the diagnostic methods currently in use need evaluation for analytical performance. objective the present study tries to evaluate the hematological indices, serologic markers of inflammation and symptoms in patients suspected of bruce...

OBJECTIVES To identify Chlamydia trachomatis via polymerase chain reaction and a direct fluorescent antibody assay in patients with vernal keratoconjunctivitis while comparing the efficacies of both tests for detecting Chlamydia trachomatis in these conditions. METHODS Conjunctival scraping samples were obtained from 177 patients who were divided into two groups: a vernal keratoconjunctivitis...

background: human enteroviruses (evs) may have a role as a possible risk factor in the pathogenesis of mi. the aim of this study was to evaluate the presence of enterovirus genomic rna in peripheral blood samples of patients with acute myocardial infarction (mi). methods: we investigated the presence of enterovirus genomic rna in the peripheral blood of 115 patients with acute mi hospitalized i...

background: vasopressin type 2 receptor (v2r) plays an important role in the water reabsorption in the kidney collecting ducts. v2r is a g protein coupled receptor (gpcr) and the triplet of amino acids aspartate-arginine-histidine (drh) in this receptor might significantly influence its activity similar to other gpcr. however, the role of this motif has not been fully confirmed. therefore, the ...

results no borrelia dna was detected by pcr in skin lesions of patients with morphea. patients and methods sixty six patients with morphea were prospectively included in the present study. for each patient, formalin-fixed, paraffin-embedded tissue blocks of skin lesion biopsies were examined for borrelia spp. dna using pcr. objectives we aimed to find the frequency of borrelia in morphea lesion...

conclusions this study suggests that the vaca pcr can be very useful in diagnosis, treatment, and follow-up of patients with gastritis and pud. the pcr assay and clo test can complement and confirm each other in identification and eradication of cancer-related strains of h. pylori. the exclusion of patients with suspected achlorhydria from the study is likely a reason for the lack of false posi...