Although the pectin content of tomatoes is relatively low in comparison with other fruits (Money & Christian, 1950), the activity of pectinesterase (pectin pectyl-hydrolase, EC 3.1.1.11) is particularly high (Kertesz, 1938). Softening of the fruit takes place relatively rapidly during ripening (Hobson, 1959), and the classical mechanism by which the effect is usually explained assumes the solub...

Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 degrees C and had optimum activity at pH 5 and 50 degrees C. Optimal conditions for PG extraction were: one time extrac...

Background and aims: Colletotrichum graminicola (Glomerellaceae, Glomerellales), the causal agent of maize (Zea mays) anthracnose, as many other fungal pathogens, relies on its battery cell wall degrading enzymes (CWDEs) to make way through walls host, thus production these is considered a virulence factor. Since this disease relatively new in Argentina, little known about capacity CWDEs native...

Pectin lyase and polygalacturonase production by newly isolated Penicillium viridicatum strain Rfc3 was carried out by means of solid state fermentation using orange bagasse, corn tegument, wheat bran and mango and banana peels as carbon sources. The maximal activity value of polygalacturonase (Pg) (30U.g) was obtained using wheat bran as carbon source while maximal pectin lyase (Pl) (2000 U.g)...

Nine Aspergillus niger isolates differing in virulence were assayed for polygalacturonase (PG) content and isoenzyme pattern in spores, liquid cultures and inoculated onion seeds of cultivar ‘Bianca Agostana’. The isolates differed in PG production quantitatively and in terms of isoenzyme pattern. Isolates An1 and An5, characterised by low PG production in liquid culture and low virulence, lack...

Polygalacturonase (PG) activity found in the salivary gland apparatus of the western tarnished plant bug (WTPB, Lygus hesperus Knight) has been thought to be the main chemical cause of the damage inflicted by this mirid when feeding on its plant hosts. Early viscosity and thermal stability studies of the PG activity in L. hesperus protein extracts were difficult to interpret. Thus, it has been ...

In this paper, the saccharification process of okara fiber with pectinase, xylanase and cellulase was investigated as a preliminary attempt to effectively utilize okara fiber. The solubilization of okara fiber was raised in proportion to the number of enzymes used; that is, by a single enzyme, two enzymes and three enzymes, in that order. The saccharification of okara fiber was much more comple...

Physiological processes characteristic of ripening in tissues of intact tomato fruit (Lycopersicon esculentum Mill.) were examined in excised pericarp discs. Pericarp discs were prepared from mature-green tomato fruit and stored in 24-well culture plates, in which individual discs could be monitored for color change, ethylene biosynthesis, and respiration, and selected for cell wall analysis. W...