Ferromagnetic carbon-coated cobalt nanoparticles have been ligated by a covalent or non-covalent strategy with dendrons or polymers bearing amino-, hydroxy- or polyether-functionalities, aiming at synthesizing materials that are dispersible in aqueous solutions, being a prerequisite for applications in biomedicine. Most efficiently for this purpose proved to be a covalently anchored polyethylen...

Polyethyleneimine (PEI)-alginate (Alg) nanoparticle (NP) is a safe and effective vector for delivery of siRNA or DNA. Recent studies suggest that autophagy is related to cytotoxicity of PEI NPs. However, contribution of autophagy to degradation of PEI-Alg NPs remains unknown. CD34+VEGFR-3+ endothelial progenitor cells isolated from rat bone marrow were treated with 25 kDa branched PEI modified ...

Maintaining cells in culture is essential for studying many aspects of cell biology and physiology. Cell culturing is dependent on proper anchorage of cells to the growth surfaces. For most cell lines, and especially for post-mitotic neurons, coated tissue culture plates are prerequisite for seeding. The most commonly used coating reagents are positively charged polymers such as poly-L-lysine o...

A novel method to prepare microstructured polymer brushes using TiO2 nanoparticles and photocatalytic microcontact printing is described. It is shown that ethanol amine can be polymerized to linear polyethyleneimine (PEI) driven by the photocatalytic action of TiO2. Upon UV irradiation during microcontact printing of ethanol amine with a stamp coated with TiO2 nanoparticles, patterned polymer b...

Treatment of diseases on the molecular level by genetic material is limited by effective delivery mechanisms. We focused on the synthesis of a pH-sensitive gene delivery vehicle based on dimethylaminoethyl methacrylate (DMAEMA) with tunable swelling, cross-linking density, and DNA release kinetics within the endosomal pH range. Our strategy, which utilized a single step for DNA encapsulation, e...

Synthesizing azoles from ortho-substituted anilines, CO2 and H2 has proved difficult due to the low nucleophilicity of which hinders their N-formylation, i.e., first step reaction. Herein, we demonstrate that R3SnX Lewis acids (LA), N-methylmorpholine (NMM) or DBU polyethyleneimine (PEI) N-formylmorpholine efficiently catalyse synthesis benzimidazole other by reductive coupling nucleophilic ami...

The direct deposition of polyethyleneimine (PEI), a weak polycation with large content amino groups, onto sand fractions different sizes (F70, F100, F200, and F355), resulted in versatile core-shell sorbents for water cleaning. Herein, PEI the polyanion poly(acrylic acid) (PAA) were directly precipitated as an nonstoichiometric polyelectrolyte complex ([PEI]:[PAA] = 2:1) surface followed by cro...

Human turbinate mesenchymal stromal cells (hTMSCs) are novel stem cells derived from nasal inferior turbinate tissues. They are easy to isolate from the donated tissue after turbinectomy or conchotomy. In this study, we applied hTMSCs to a nonviral gene delivery system using polyethyleneimine (PEI) as a gene carrier; furthermore, the cytotoxicity and transfection efficiency of hTMSCs were evalu...

Doxorubicin-loaded micelles were prepared from a copolymer comprising cholic acid (CA) and polyethyleneimine (PEI) for the delivery of antitumor drugs. The CA-PEI copolymer was synthesized via pairing mediated by N,N'-dicyclohexylcarbodiimide and N-hydroxysuccinimide using dichloromethane as a solvent. Fourier transform infrared and nuclear magnetic resonance analyses were performed to verify t...

This study developed novel triblock pH-responsive polymeric micelles (PMs) using cholic acid-polyethyleneimine-poly-L-arginine (CA-PEI-pArg) copolymers. PEI provided pH sensitivity, while the hydrophilic cell-penetrating pArg peptide promoted cellular PM internalization. The copolymers self-assembled into PMs in aqueous solution at above the critical micelle concentration (2.98 × 10-7 M) and en...