The equilibrium adsorption of bovine serum albumin (BSA) on poly-3-hydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) surfaces was studied at different solution concentrations. data were fitted to the Langmuir Freundlich models. protein behaviour further investigated by analysing conformation surface adsorbed BSA using Fourier transform infrared (FTIR) spectroscopy wi...

BACKGROUND Poly-beta-hydroxybutyrate (PHB) mobilization in bacteria has been proposed as a mechanism that can benefit their host for survival under stress conditions. Here we reported for the first time that a stress-induced system enabled E. coli, a non-PHB producer, to mobilize PHB in vivo by mimicking natural PHB accumulation bacteria. RESULTS The successful expression of PHB biosynthesis ...

Batch culture of Ralstonia eutropha using the recycled gas closed circuit culture system was conducted in order to develop a practical fermentation system for industrial autotrophic culture for poly (hydroxybutrate) production. The gas phase of the culture system consisted of substrate gas so that gases in this culture could be recycled as long as the amount of the gas consumed would be repleni...

Factors influencing the utilization of ketone bodies by mouse adipose tissue in vitro were studied. Epididymal fat pads can oxidize DL-Beta-hydroxybutyrate-3-(14)C and acetoacetate-3-(14)C to (14)CO(2) as well as convert these compounds to fatty acid-(14)C. An increased output of (14)CO(2) from Beta-hydroxybutyrate-3-(14)C was noted in response to glucose plus insulin, succinate, oxaloacetate, ...

Forearm muscle metabolism was studied in eight obese subjects after an overnight, 3 and 24 day fast. Arterio-deep-venous differences of oxygen, carbon dioxide, glucose, lactate, pyruvate, free fatty acids, acetoacetate, and beta-hydroxybutyrate with simultaneous forearm blood flow were measured. Rates of metabolite utilization and production were thus estimated. Oxygen consumption and lactate a...

Many bacteria are naturally capable of accumulating biopolyesters composed of 3-hydroxy fatty acids as intracellular inclusions, which serve as storage granules. Recently, these inclusions have been considered as nano-/microbeads with surface-attached proteins, which can be engineered to display various protein-based functions that are suitable for biotechnological and biomedical applications. ...

Parenchymal cells isolated from the liver of 24 h fasted rats were incubated with 65 mM R,S-1-3H -ethanol plus 3 mM pyruvate as substrates in the absence and presence of 1.7 mM 4-methylpyrazole. Metabolite levels and the time-course of the transfer of tritum from ethanol to lactate and beta-hydroxybutyrate was measured during the first 15 min of ethanol metabolism. The time-course of the loss o...

Feast and famine cycles are common in activated sludge wastewater treatment systems, and they select for bacteria that accumulate storage compounds, such as poly-beta-hydroxybutyrate (PHB). Previous studies have shown that variations in influent substrate concentrations force bacteria to accumulate high levels of rRNA compared to the levels in bacteria grown in chemostats. Therefore, it can be ...