Electromigration capillary methods are promising techniques in proteomics and they are still under research. We used a partial filling approach, i.e. a combination of gel and non-gel separation mechanisms in a single dimension. We tried using an interesting gel, Pluronic F 127, which can be considered as a surfactant capable of self-association both with isotropic and anisotropic gels. The Plur...

Calcium phosphates like hydroxyapatite (HA), beta-tricalcium phosphate (beta-TCP), and their mixtures (biphasic calcium phosphates; BCP) are used clinically to repair bone defects. These materials can be difficult to handle and have no inherent biological activity. Handling properties of other bone substitute materials have been improved by combining them with an inert carrier such as Pluronic ...

Because of high occurrence of side effects of conventional anticancer drugs, new therapies have been widely studied, such as the use of non-usual molecules and the association with nanomaterials. In this context, sildenafil is a molecule that has been lately considered as a co-adjuvant in cancer therapy because it inhibits an enzyme with a potential role in tumor progression, phosphodiesterase-...

Background: Although microemulsion-based nanoparticles (MEs) may be useful for drug delivery or scavenging, these benefits must be balanced against potential nanotoxicological effects in biological tissue (bio-nano interface). We investigated the actions of assembled MEs and their individual components at the bio-nano interface of thrombosis and hemolysis in human blood. Methods: Oil-in-water M...

We developed a two fluid atomizer to spray mammalian cells suspended in phosphate buffered saline (PBS)-Pluronic F-127 polymer solutions. We used the device to spray suspensions of bovine articular chondrocytes and porcine tracheal epithelial cells, in either PBS or the polymer solutions, and studied their viability and growth kinetics. Under the operating conditions examined in this study, the...

Sumatriptan succinate is an agonist for a vascular 5-hydroxytryptamine (5-HT)1 receptor subtype (probably a member of the 5-HT1D family). It does not have significant affinity for the remaining 5-HT receptors. It does not have affinity for alpha1, alpha2 or beta-adrenergic, dopamine1, dopamine2, muscarinic or benzodiazepine receptors. The objective of the study was to evaluate the in vitro tran...

Aggregation can have a number of deleterious effects on biotherapeutics including the loss of efficacy, the induction of unwanted immunogenicity, altered pharmacokinetics, and reduced shelf life. Aggregation is ameliorated by the inclusion of surfactants in biotherapeutics formulations, typically non-ionic polymeric ether surfactants. The most commonly used examples are Tween 20 (Polysorbate 20...