نتایج جستجو برای: pluripotency specific

تعداد نتایج: 1040520  

2014
Hsu-Hsin Chen Maaike Welling Donald B. Bloch Javier Muñoz Edwin Mientjes Xinjie Chen Cody Tramp Jie Wu Akiko Yabuuchi Yu-Fen Chou Christa Buecker Adrian Krainer Rob Willemsen Albert J. Heck Niels Geijsen

The scarcity of primordial germ cells (PGCs) in the developing mammalian embryo hampers robust biochemical analysis of the processes that underlie early germ cell formation. Here, we demonstrate that DAZL, a germ cell-specific RNA binding protein, is a robust PGC marker during in vitro germ cell development. Using Dazl-GFP reporter ESCs, we demonstrate that DAZL plays a central role in a large ...

Journal: :Cell stem cell 2016
Jonathan A Beagan Thomas G Gilgenast Jesi Kim Zachary Plona Heidi K Norton Gui Hu Sarah C Hsu Emily J Shields Xiaowen Lyu Effie Apostolou Konrad Hochedlinger Victor G Corces Job Dekker Jennifer E Phillips-Cremins

Pluripotent genomes are folded in a topological hierarchy that reorganizes during differentiation. The extent to which chromatin architecture is reconfigured during somatic cell reprogramming is poorly understood. Here we integrate fine-resolution architecture maps with epigenetic marks and gene expression in embryonic stem cells (ESCs), neural progenitor cells (NPCs), and NPC-derived induced p...

Journal: :Cell reports 2014
Li-Ying Sung Wei-Fang Chang Qian Zhang Chia-Chia Liu Jun-Yang Liou Chia-Chun Chang Huan Ou-Yang Renpeng Guo Haifeng Fu Winston T K Cheng Shih-Torng Ding Chuan-Mu Chen Maja Okuka David L Keefe Y Eugene Chen Lin Liu Jie Xu

Haplo-insufficiency of telomerase genes in humans leads to telomere syndromes such as dyskeratosis congenital and idiopathic pulmonary fibrosis. Generation of pluripotent stem cells from telomerase haplo-insufficient donor cells would provide unique opportunities toward the realization of patient-specific stem cell therapies. Recently, pluripotent human embryonic stem cells (ntESCs) have been e...

Journal: :Cell 2011
Yick W. Fong Carla Inouye Teppei Yamaguchi Claudia Cattoglio Ivan Grubisic Robert Tjian

The transcriptional activators Oct4, Sox2, and Nanog cooperate with a wide array of cofactors to orchestrate an embryonic stem (ES) cell-specific gene expression program that forms the molecular basis of pluripotency. Here, we report using an unbiased in vitro transcription-biochemical complementation assay to discover a multisubunit stem cell coactivator complex (SCC) that is selectively requi...

2018
Megan L Rasmussen Natalya A Ortolano Alejandra I Romero-Morales Vivian Gama

The core transcriptional network regulating stem cell self-renewal and pluripotency remains an intense area of research. Increasing evidence indicates that modified regulation of basic cellular processes such as mitochondrial dynamics, apoptosis, and cell cycle are also essential for pluripotent stem cell identity and fate decisions. Here, we review evidence for Wnt regulation of pluripotency a...

Journal: :Molecular oncology 2015
George Notas Vassiliki Pelekanou Marilena Kampa Konstantinos Alexakis Stelios Sfakianakis Aggelos Laliotis John Askoxilakis Eleftheria Tsentelierou Maria Tzardi Andreas Tsapis Elias Castanas

Tamoxifen is the treatment of choice in estrogen receptor alpha breast cancer patients that are eligible for adjuvant endocrine therapy. However, ∼50% of ERα-positive tumors exhibit intrinsic or rapidly acquire resistance to endocrine treatment. Unfortunately, prediction of de novo resistance to endocrine therapy and/or assessment of relapse likelihood remain difficult. While several mechanisms...

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