BACKGROUND AND OBJECTIVES Storage lesions may prevent transfused platelets to respond to agonists and arrest bleeding. The aim of this study was to evaluate and quantify the capacity of platelet activation during storage using flow cytometry and new markers of platelet activation. MATERIALS AND METHODS Activation responses of platelets prepared by apheresis were measured on days 1, 5, 7 and 1...

Platelet concentrates can be stored for five days at 22 C. Our objective was to increase the storage of platelets to 7 days by decreasing the storage temperature. Lower temperature may minimize chances of bacterial proliferation and also maintain platelet functions to optimum level. The study sample iincluded 25 blood donors of both sex in State Blood Bank, Chhatrapati Shahuji Maharaj Medical U...

A quantitative study of various aspects of platelet function was carried out in eight patients with typical hairy-cell leukaemia (HCL). In at least two patients platelet aggregation was convincingly reduced to more than one aggregating agent (ADP, adrenaline, collagen, thrombin, and ristocetin). Granular storage capacity for {(14)C} 5-HT was reduced in five of the six patients tested. The two p...

Platelet concentrates were prepared at twice the normal concentration and stored at room temperature for 7 days in either standard bags (controls) or bags to which 1 or 2 g of Amberlite resin beads charged with dibasic phosphate had been added. The resin beads served as a buffer system by providing a "slow release" form of phosphate ions as well as by binding CO2 produced during platelet metabo...

Platelet concentrates were prepared for in vitro storage in either Fenwal PL-732 or Cutter CLX platelet packs. The units were stored at 22 degrees C for seven days with either horizontal or tumbler agitation. Measurement of pH, hypotonic shock response and serotonin uptake indicated in vitro viability was well maintained during 5-7 days storage using either type of pack with either mode of agit...

Citrated platelet-rich plasma (PAP) was kept at cold temperatures or room temperature. After 4 hr or more at these temperatures, the PRPs were warmed 1 hr at 37#{176}C. This prevents the spontaneous aggregation seen in chilled PRP that is stirred immediately after warming. Platelet aggregation in response to connective tissue (CT), epinephrine, and adenosine diphosphate (ADP) was considerably g...

BACKGROUND Platelet transfusion is an essential part of the treatment of a variety of conditions such as thrombocytopenia and qualitative platelet disorders. As indicated in previous reports, during in vitro storage, platelets undergo morphological and physiological changes collectively known as the platelet storage lesion. Apoptosis is a programmed process of cell death, which has been conside...