We evaluated the variability in the amount of protein adsorbed onto the surface of individual wells of an assortment of microtitre plates by use of procedures involving enzyme-protein conjugates. Coefficients of variation in adsorbed protein ranged from 5.2 to 29.5%. Microtitre plates show a distinct "edge effect"; wells at the edges of a plate adsorb more protein than those in the interior, wh...

This study describes a novel fabrication method of a multi-pad paper plate (MP) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwi...

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADM in equine serum, plasma and other biological fluids. Reagents Quantity Reagents Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard 2 Standard Diluent 1×20mL Detection Reagent A 1×120μL Assay Diluent A 1×12mL Detection Reagent B 1×120μL Assay Diluent B 1×12mL TMB Substr...

The mechanical response and fracture of metal sandwich panels subjected to multiple impulsive pressure loads (shocks) were investigated for panels with honeycomb and folded plate core constructions. The structural performance of panels with specific core configurations under multiple impulsive pressure loads is quantified by the maximum transverse deflection of the face sheets and the core crus...

We examine the elastic shear waves generated in a thin pre-stressed elastomer layer that is sandwiched between two relatively thick steel plates and is subjected to an elastic shear wave traveling in one of the steel plates. The elastomer layer has been deformed in uni-axial strain in advance, producing in the layer very large axial and lateral compressive stresses of the order of its bulk modu...

An analytical solution for a sandwich circular FGM plate coupled with piezoelectric layers under one-dimension heat conduction is presented in this paper. A nonlinear static problem is solved first to determine the initial stress state and pre-vibration deformations. By adding an incremental dynamic state to the pre-vibration state, the differential equations are derived. The role of thermal en...

In this paper, layerwise theory (LT) along with the first, second and third-order shear deformation theories (FSDT, SSDT and TSDT) are used to determine the stress distribution in a simply supported square sandwich plate subjected to a uniformly distributed load. Two functionally graded (FG) face sheets encapsulate an elastomeric core while two epoxy adhesive layers adhere the core to the face ...

This work examines the performance of composite panels when subjected to underwater impulsive loads. The scaled fluid–structure experimental methodology developed by Espinosa and co-workers was employed. Failure modes, damage mechanisms and their distributions were identified and quantified for composite monolithic and sandwich panels subjected to typical blast loadings. The temporal evolutions...

Nowadays, the floating production storage and offload system (FPSO) is one of most common platform types for offshore oil production. The traditional arrangement stiffened panels creates obstacles automated cleaning inspections by remote devices. This paper summarizes results an initial study design construction FPSO hulls with SPS in order to overcome this problem. main goal have walls bottom ...