نتایج جستجو برای: plasmid construction
تعداد نتایج: 242846 فیلتر نتایج به سال:
A plasmid vector pBK2 was tested for maintenance in Corynebacterium acetoacidophilum and found to be 100% stable for 90 generations. In co-culture experiments in absence of any selection pressure it was able to cause washout of plasmid free cells. Deletion mutagenesis of the plasmid was done to identtfi the regions of DNA responsible for its stable maintenance. This led to the identification of...
The Yeast Knockout (YKO) collection has provided a wealth of functional annotations from genome-wide screens. An unintended consequence is that 76% of gene annotations derive from one genotype. The nutritional auxotrophies in the YKO, in particular, have phenotypic consequences. To address this issue, 'prototrophic' versions of the YKO collection have been constructed, either by introducing a p...
افزایش dna به محلول لیگاند شیف باز موجب کاهش شدت جذب در اسپکتروفتومتری لیگاند ، افزایش ویسکوزیته محلول، افزایش در طیفهای فلورسنس و تغییرات کانفورماسیونی شدید در طیف دورنگ نمایی چرخشی dna شده است. این نتایج نشان دادند که این لیگاند شیف باز میتواند به طریق intercalation با dna برهمکنش بدهد. نتایج ژل الکتروفورز نیز نشان دادند که این لیگاند قادر به ایجاد cleavage در puc 18 plasmid dna می باشد. ا...
A 3.2 kb plasmid pCR2 was isolated from Corynebcterium renale which harbours four cryptic plasmids. This plasmid was used for the construction of a range of cloning and expression vectors for E.co/i. This previously uncharacterized plasmid was first sequenced by cloning its fragments into pUC19. The complete nucleotide sequence thus obtained was annotated and submitted to GENEBANK (Accession No...
UNLABELLED Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic the type I adenine methylation profiles of ...
conclusions: e. coli herb-resistance plasmid can replicate and be expressed in s. aureus. results: the identified 45 kb herb-resistance plasmid could be transferred from e. coli cp9 isolates to e. coli dh5α. as a consequence e. coli dh5α transconjugant mic increased from 0.0125 g/ml to 0.25 g/ml. the plasmid was easily transferred from e. coli cp9 strain to s. aureus rn450rf with a mean transfe...
A conjugative plasmid with potential usefulness for vaccine strains was constructed. In the first step, a 5.9-kilobase DNA segment containing the two loci for the A and B subunits of heat-labile enterotoxin with a mutation in the gene for the A subunit was joined to the cloning vehicle pGA22, generating the nonconjugative plasmid pPMC4 with genes for resistance to tetracycline and chloramphenic...
BACKGROUND Plasmid-less, engineered Bacillus strains have several advantages over plasmid-carrier variants. Specifically, their stability and potential ecological safety make them of use in industrial applications. As a rule, however, it is necessary to incorporate many copies of a key gene into a chromosome to achieve strain performance that is comparable to that of cells carrying multiple cop...
BACKGROUND Construction of plasmids is crucial in modern genetic manipulation. As of now, the common method for constructing plasmids is to digest specific DNA sequences with restriction enzymes and to ligate the resulting DNA fragments with DNA ligase. Another potent method to construct plasmids, known as gap-repair cloning (GRC), is commonly used in the budding yeast Saccharomyces cerevisiae....
background: echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection that occurs by the larval stages of taeniid cestodes of the genus echinococcus . iran is known as endemic region for this infection in the world. vaccination has been considered as a good prevention method for this disease. recombinant vaccines containing eg95 protein, against e. granulosus , has shown a high d...
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