The cholesteryl ester content of plasma low density lipoproteins (LDL) in monkeys has previously been shown to be related to the rate of hepatic cholesterol secretion and cholesteryl ester content of newly secreted lipoproteins in the isolated perfused liver. In the present studies, African green monkeys were fed diets containing cholesterol and 40% of calories as either butter or safflower oil...

We studied the ability of lipid-transfer factors in plasma to promote transfer, to endogenous lipoproteins, of [3H]cholesteryl ester from high-density lipoprotein (HDL) covalently bound to Sepharose 4B beads. After incubation for 2 h at 37 degrees C, 12 to 14% of the [3H]cholesteryl ester had been transferred to the lipoproteins of the plasma, in the proportions 57% to HDL and 43% to low- and v...

Among the apoA-I-containing lipoproteins isolated by selected-affinity immunosorption from human serum and plasma, we have identified a subpopulation which, unlike the bulk of high density lipoproteins, has pre-beta electrophoretic mobility. This pre-beta subpopulation can be observed directly in fresh plasma by immunoelectrophoresis. It contains phospholipid and free and esterified cholesterol...

Modifications of plasma lipoprotein structure and function resulting from in vivo post-translational nonenzymatic glycosylation may play a role in the premature atherosclerosis of patients with diabetes mellitus. This report describes the generation and characterization of six unique murine monoclonal antibodies that bind glucosylated human plasma lipoproteins, but do not react with normal plas...

We isolated and characterized immunoreactive apolipoprotein B (apoB)-containing lipoproteins from human atherosclerotic plaque and plasma to determine whether very-low-density lipoprotein (VLDL) can enter and become incorporated into the atherosclerotic lesion and how plaque apoB-containing lipoproteins differ from apoB-containing lipoproteins isolated from plasma. Atherosclerotic plaques were ...

Psoriasis is an epidermal disease characterized by chronic relapsing papillon squamous erythematous plaques which may develop in the skin of elbows, knees and extensor surfaces. The cause of psoriasis is unknown. The primary etiologic factor may be related to excessive uncontrolled synthesis is resulting from changes in (1) the function of growth factors ( EGF),(2) presence of proto- oncogenes,...

Background: Preeclampsia is one of the most common complications of pregnancy. It is the cause of high morbidity and mortality for mothers and fetuses, especially in developing countries. The etiology of preeclampsia is unknown. One of the hypothesis on pathophysiology of preeclampsia is dyslipidemia. The aim of this study was to determine maternal plasma levels of lipoproteins in patients with...

A marked elevation of plasma lipoproteins is found in rats with nephrotic syndrome induced by puromycin aminonucleoside (Marsh and Sparks 1979a; Gherardi, Vecchia and Calandra 1980; Gherardi and Calandra 1982). Although the hepatic production of lipoproteins is increased in nephrotic rats (Shafrir and Brenner 1979; Calandra, Gherardi, Fainaru, Guaitani and Bartofek 1981) the mechanism of plasma...

Based on a previously described technique [Clin. Chem. 19, 737 (1973)] of precipitating plasma lipoproteins with polyanions after their electrophoretic separation in gels, a new method is presented for measuring normal plasma lipoproteins densitometrically. The method is fast and easy; the CV for beta-, pre-beta-, and alpha-lipoproteins was less than 5% in one series. Results are linearly relat...

Fast lipoprotein chromatography (FLPC) is a novel method for quantifying lipoproteins. Plasma proteins are separated by fast-flow gel filtration. Lipoproteins are detected by post-column derivatization with an enzymatic cholesterol reagent. FLPC resolves very-low-, low-, and high-density lipoproteins (VLDL, LDL, and HDL, respectively) and completely separates apolipoprotein Al- and apolipoprote...