نتایج جستجو برای: plaque reduction neutralization test

تعداد نتایج: 1303862  

Journal: :Clinical and vaccine immunology : CVI 2009
W W Shanaka I Rodrigo D C Alcena Z Kou T J Kochel K R Porter G Comach R C Rose X Jin J J Schlesinger

Sera from patients involved in a Peruvian outbreak of dengue virus serotype 1 infection cross-neutralized the American genotype of dengue virus serotype 2 up to 100-fold more efficiently than they did the virulent Asian genotype of dengue virus serotype 2, as determined by a plaque reduction neutralization test (PRNT) with CV-1 fibroblasts modified to express human Fcgamma receptor CD32. The co...

Journal: :Journal of clinical microbiology 2002
Pei-Yun Shu Li-Kuang Chen Shu-Fen Chang Yi-Yun Yueh Ling Chow Li-Jung Chien Chuan Chin Hui-Hua Yang Ting-Hsiang Lin Jyh-Hsiung Huang

An NS1 serotype-specific indirect enzyme-linked immunosorbent assay (ELISA) was developed to differentiate primary and secondary dengue virus infections and serotypes of primary dengue virus infection. For this report, we carried out retrospective seroepidemiologic studies on serum samples collected from residents of Liuchiu Hsiang, Pingtung County, an isolated island in southern Taiwan during ...

Journal: :Virology 2015
Xinsheng Zhang Olivia L Wallace Arban Domi Kevin J Wright Jonathan Driscoll Omu Anzala Eduard J Sanders Anatoli Kamali Etienne Karita Susan Allen Pat Fast Jill Gilmour Matt A Price Christopher L Parks

Serum was analyzed from 146 healthy adult volunteers in eastern Africa to evaluate measles virus (MV) and canine distemper virus (CDV) neutralizing antibody (nAb) prevalence and potency. MV plaque reduction neutralization test (PRNT) results indicated that all sera were positive for MV nAbs. Furthermore, the 50% neutralizing dose (ND50) for the majority of sera corresponded to antibody titers i...

Journal: :Transfusion 2021

Background Convalescent plasma products are a potential passive immunotherapy for Coronavirus disease 2019 (COVID-19) disease. Various approaches have been utilized to determine the concentration of Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-neutralizing antibodies in products. The Canadian Blood Services used Plaque Reduction Neutralization Test 50 (PRNT50)-generated values q...

2017
Andres Moreira-Soto Manoel Sarno Celia Pedroso Eduardo Martins Netto Alexandra Rockstroh Estela Luz Marie Feldmann Carlo Fischer Fernanda Anjos Bastos Beate M Kümmerer Xavier de Lamballerie Christian Drosten Sebastian Ulbert Carlos Brites Jan Felix Drexler

Reliable diagnosis of congenital Zika virus (ZIKV) infection is challenging. Here, we assessed ZIKV-specific neutralizing antibodies in 28 mothers of children with microcephaly (cases) and 122 controls from northeastern Brazil using plaque reduction neutralization tests. ZIKV-specific antibody titers were significantly higher in cases than in controls (t test, P < .0001). We identified a putati...

Journal: :Vector borne and zoonotic diseases 2011
Diana Lupulovic Miguel A Martín-Acebes Sava Lazic Julio Alonso-Padilla Ana-Belén Blázquez Estela Escribano-Romero Tamas Petrovic Juan-Carlos Saiz

West Nile virus (WNV), the most widely distributed flavivirus worldwide, has lately reemerged in Europe, causing worrisome outbreaks in humans and horses. Serological analysis by enzyme-linked immunoassay and plaque reduction neutralization test showed for the first time in Serbia that 12% of 349 horses presented specific neutralizing WNV antibodies, which in one case also cross-neutralized Usu...

Journal: :The New England journal of medicine 2016
Abigail R A Aiken James G Scott Rebecca Gomperts James Trussell Marc Worrell Catherine E Aiken

n engl j med 375;4 nejm.org July 28, 2016 2. Petersen LR, Jamieson DJ, Powers AM, Honein MA. Zika virus. N Engl J Med 2016; 374: 1552-63. 3. Lanciotti RS, Kosoy OL, Laven JJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis 2008; 14: 1232-9. 4. Russell PK, Nisalak A. Dengue virus identification by the plaque reducti...

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