Sclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant's cell-wall components. Functional analyses of the genes that encode CWDEs will help explain the mechanisms of growth and pathogenicity of S. sclerotiorum. Here, we isolated and ch...

A molecular diagnostic system using single nucleotide polymorphisms (SNPs) was developed to identify four Sclerotinia species: S. sclerotiorum (Lib.) de Bary, S. minor Jagger, S. trifoliorum Erikss., and the undescribed species Sclerotinia species 1. DNAs of samples are hybridized with each of five 15-bp oligonucleotide probes containing an SNP site midsequence unique to each species. For addit...

Sclerotinia sclerotiorum is a phytopathogenicfungus that attacks more than 400 plant species of them medicinal plant. In this study the extract obtained from basal stalk rot of Helianthus annuus L., attacked by S. sclerotiorum, was subjected to the analysis of FTIR to identify the presence of beta-glucan. FT-IR spectrum showed four ranges of bands in 890 cm-1, two overlap band in 1047 and 1078 ...

Five herbicides commonly used in rapeseed crop, canola (Brassica napus var Olifera) fields in Iran were studied with an eye to their antifungal activities against six isolates of Trichoderma species and also the phytopathogenic fungus Sclerotinia sclerotiorum. The doses tested were the same as are recommended for applications in canola crops. The results showed that the dinitro aniline herbicid...

Sclerotinia sclerotiorum (Lib.) de Bary, the causal organism of stem rot of Brassica and over 500 host plants is distributed worldwide. Sclerotinia rot is menace to cultivation of oilseed Brassica crops in the world. Infection occurs on leaves, stems and pods at different developmental stages, causing seed yield losses of up to 80%, as well as significant reductions in oil content and quality. ...

Plant diseases are a threat for global food safety. Allium obliquum, Allium fistulosum, Allium ursinum, Aloe vera, Berberis vulgaris and Chelidonium majus plant extracts were obtained by the repercolation method and the antifungal activity was determined by the agar-dilution assay against Apergillus niger, Botrytis cinerea, Botrytis paeoniae, Fusarium oxysporum f. sp. gladioli, Fusarium oxyspor...

Mitogen-activated protein kinases (MAPKs) play a central role in transferring signals and regulating gene expression in response to extracellular stimuli. An ortholog of the Saccharomyces cerevisiae cell wall integrity MAPK was identified in the phytopathogenic fungus Sclerotinia sclerotiorum. Disruption of the S. sclerotiorum Smk3 gene severely reduced virulence on intact host plant leaves but...

Sclerotinia species are important fungal pathogens of a wide range of crops and wild host plants. While the biology and population structure of Sclerotinia sclerotiorum has been well-studied, little information is available for the related species S. subarctica. In this study, Sclerotinia isolates were collected from different crop plants and the wild host Ranuculus ficaria (meadow buttercup) i...

Vicilins (7S globulins) are seed storage proteins and constitute the main protein family in legume seeds, particularly in narrow-leafed lupin (Lupinus angustifolius L.; NLL), where seven vicilin genes, called β1- to β7-conglutin have been identified. Vicilins are involved in germination processes supplying amino acids for seedling growth and plant development, as well as in some cases roles in ...

Microbial opsins play a crucial role in responses to various environmental signals. Here, we report that the microbial opsin homolog gene sop1 from the necrotrophic phytopathogenic fungus Sclerotinia sclerotiorum was dramatically up-regulated during infection and sclerotial development compared with the vegetative growth stage. Further, study showed that sop1 was essential for growth, sclerotia...