Back Ground: The plant tissue culture technique is one of the biotechnologies that meansisolating a cell, or organ, sterilizing it and cultivating on sterile artificial foodmedia under conditions completely free pathogens, then transplanted part developsinto complete similar to original from which was taken. Under controlledenvironmental temperature, humidity lighting, as defined by others ster...

Biotechnology has been globally accepted as one of the important tools for direct application in agriculture. Agricultural biotechnology includes plant tissue culture (PTC), applied microbiology, and molecular biology contributing to production crops with improved food, feed, fiber fuel. The technique PTC is well translated from ‘concept’ ‘commercialization’. It flourishing multidirectional gro...