Background: The use of conventional stains including Giemsa, Wright and Leishman have become an essential tool for differential diagnosis of cells in peripheral blood. Aims: The aim of the study was to develop a new combination of Leishman-Giemsa (L&G;) stain for avian blood smears and to compare its efficacy with conventional staining methods. Methods:<...

Her haemoglobin concentration was 5.4 g/dl, the mean cell volume 101 fl, and the corrected reticulocyte count 1.4%. There were oval macrocytes on the blood smear. Red cell folate and serum vitamin B12 levels were normal. Her bone marrow aspirate was hypercellular with predominantly erythroid hyperplasia and trilineage megaloblastic dysplasia. An iron stain showed numerous ringed sideroblasts (2...

In a case of neuroblastoma the presence of an abnormal blood constituent was suspected from the raised erythrocyte sedimentation rate, sludging of the red cells, marked rouleaux formation, an atypical Leishman stain, increased plasma viscosity, and a distorted protein electrophoresis pattern. The abnormal constituent was shown to be a mucopolysaccharide which was either hyaluronic acid or chond...

We synthesized a series of Seoul-Fluor-based lipid droplet bioprobes with a linear range of lipophilicity and identified SF44 and SF58 as SF-based LD bioprobes in microalgae for biofuel research as well as in mammalian cells. Unlike Nile Red, SF-based bioprobes can stain algal LDs with excellent efficiency under the non-invasive and non-cytotoxic conditions.

Cover Photomicrograph of lymphoid follicles of the tonsil of a mule deer (Odocoileus he&onus) with chronic wasting disease (CWD). The dark red staining is the prion that is associated with chronic wasting disease. Im-munohistochemical stain with monoclonal antibody 160.

Some techniques available at our laboratory were tested for their ability to aid in the morphological diagnosis of hydatid elements (Echinococcus granulosus ["Taenia echinococcus"]) isolated from cysts in humans and sheep. Unstained, methanol-fixed hooklets were fluorescent, most starkly so under violet light (excitation filter wavelength, 405 nm; long-pass filter wavelength, 495 nm). Auramine-...

BACKGROUND Oral Submucous fibrosis (OSMF) is a common potentially malignant disease, which is characterized by generalized fibrosis of the oral soft tissues. It is due to disproportion in the collagen deposition and degradation. The excess deposition of collagen results in juxta epithelial fibro elastic changes and epithelial atrophy. Neoplastic changes in the epithelium usually precede the cha...

Introduction: Skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. The aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. Methods: In this pa...

A n 80-year-old man presented with chronic pain in the epigastrium. An upper gastrointestinal endoscopy with esophageal biopsy was performed. Histopathologic sections showed Barrett’s mucosa (panel A; original magnification 320, hematoxylin and eosin stain) with marked plasmacytic infiltrate laden with numerous Russell bodies in the lamina propria (panel B; original magnification 340, hematoxyl...

The intense luminescence of the new complex Ir(ppy)(2)(pybz) (1) within the cytoplasm of live cells can be discriminated from the fluorescence of an organic stain, solely on the basis of the emission timescale {pybzH = 2-pyridyl-benzimidazole}. The protonated form of 1 displays red-shifted emission, and may be implicated in a superior uptake compared to Ir(ppy)(3).