Staphylococcal polysaccharide intercellular adhesin (PIA) is important for the development of a mature biofilm. PIA production is increased during growth in a nutrient-replete or iron-limited medium and under conditions of low oxygen availability. Additionally, stress-inducing stimuli such as heat, ethanol, and high concentrations of salt increase the production of PIA. These same environmental...

Alginate overproduction by P. aeruginosa strains, also known as mucoidy, is associated with chronic lung infections in cystic fibrosis (CF). It is not clear how alginate induction occurs in the wild-type (wt) mucA strains. When grown on Pseudomonas isolation agar (PIA), P. aeruginosa strains PAO1 and PA14 are non-mucoid, producing minimal amounts of alginate. Here we report the addition of ammo...

the effects of adenosine,5-n-ethylcarbox-amidoadenosine (neca), 2-chloroadenosine (2-ca), n6-phenyiisopropyladenos'tne (l-pia and d-pia) and n6 -cyclohexyladenos'tne (cha) were examined on the mouse isolated vas deferens. all the compounds in a concentration-dependent manner inhibited electrically induced contrac¬tions. ic of adenosine and its analogues were 13.68 ± 5.97 for ado, 0.736 ± 0.087 ...

Cost-e ective development and widespread deployment of spoken language systems is only possible if their development process is substantially supported by intelligent design tools. In this paper, we present PIA a new approach for fast prototyping of complex spoken dialog systems. Dialog structures are speci ed in a specialised fully declarative design language. Dialog design in PIA is considere...

A PCR-enhanced immunoassay (PIA) to detect enterovirus (EV) immunoglobulin M (IgM) for diagnosis of recent EV infection was recently developed. This test was compared with another EV IgM capture technique, the solid-phase reverse immunosorbent test (SPRIST). Fourteen of 43 serum samples from aseptic meningitis patients were positive by PIA, whereas 10 were positive by SPRIST. One of 39 control ...

In this paper, an efficient algorithm to find the center of the biggest circle inscribed in a given polygon is described. This work was inspired by the publication of Daniel Garcia-Castellanos & Umberto Lombardo and their algorithm [1] used to find a landmass’ poles of inaccessibility. Two more efficient algorithms were found, one of them only applicable when the problem can be described as a l...

A number of semantic desktop frameworks have been proposed that address different issues in personal information management, including the organization, manipulation, and visualization of personal data. Our approach comprises a layered multi-ontology based framework, MOSE. In this paper, we describe how the architecture of MOSE supports an application centered semantic approach to personal info...

Hemagglutination of erythrocytes is a common property of Staphylococcus epidermidis strains, which is related to adherence and biofilm formation and may be essential for the pathogenesis of biomaterial-associated infections caused by S. epidermidis. In three independent biofilm-producing, hemagglutination-positive S. epidermidis isolates, interruption of the icaADBC operon essential for polysac...

Our goal was to create a support for better PLM development target identification. A method titled ”PIA – PLM Impact Analysis” was created in order to help recognizing the most value-adding potentials. The method is supported by a MS–Excel based tool. The method was built and developed in workshops interactively with researchers and representatives from companies. So far the PIA method and tool...

Whole bacteria, isolated outer membranes, and purified protein I (PI) from one transparent (O-) and two different opaque (O+) phenotype gonococcal strains (serogroups I, II, and III; PI serotypes 1, 5, and 9b) were each treated with tolylsulfonyl phenylalanyl chloromethyl ketone-trypsin, alpha-chymotrypsin, and proteinase K. Protein IA (PIA) of strain 7122 (O-, serotype 1, serogroup I) was resi...