In the present study we determined the effect of the lectins concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed (PWM), frequently used in cell proliferation assays, on the proliferation of goat peripheral blood mononuclear cells (PBMCs). The use of a standardized Percoll gradient proved to be highly effective for the separation/enrichment of these cells, without damaging the cell mem...

Phytohemagglutinin-induced lymphocyte deoxyribonucleic acid synthesis, dermal hypersensitivity, and peripheral blood thymus-derived lymphocyte numbers were assessed in nine men with experimentally induced rubella infection. Five of these men and two additional volunteers received treatment with tilorone dihydrochloride, an antiviral drug. Response to phytohemagglutinin was not changed during ru...

Post weaning rats were fed protein deficient dies for about 6 weeks until their body weight/brain weight ratio started to decrease. Effect of such malnutrition on the plaque forming response of antibody producing spleen cells against three bacterial antigens, Vibrio cholerae, Salmonella paratyphii and Staphylococcus aureus were studied. Mitogen stimulation of spleen cells was also studied using...

In a group of Thai patients with P. falciparum acute malaria, circulating malarial antigens (CMA) were detected in 27/33 cerebral malaria (CM) cases and 31/43 noncerebral cases. Delayed cutaneous responses to phytohemagglutinin and candidin were found frequently negative in patients with CMA, especially in the CM group. Mean in vitro lymphocyte proliferative responses to lectins were lower in t...

Abstract Bats use olfactory signals to provide information of species and individual identity quality. Males two long-nosed bats, Leptonycteris curasoae L. yerbabuenae , display an odoriferous dorsal patch during the mating season that is involved in mate choice. The documented serve as indicator male quality; for instance, males with present larger testes lower ectoparasite loads. We evaluated...