MOTIVATION Phosphorylation is involved in diverse signal transduction pathways. By predicting phosphorylation sites and their kinases from primary protein sequences, we can obtain much valuable information that can form the basis for further research. Using support vector machines, we attempted to predict phosphorylation sites and the type of kinase that acts at each site. RESULTS Our predict...

G protein-coupled receptor kinaseinteracting protein 1 (GIT1) was originally identified as an ADP ribosylation factor GTPase-activating protein (ARF-GAP) that binds Gprotein-coupled receptor kinases (GRKs) and regulates membrane trafficking (Premont et al., 1998). Subsequent studies have shown a much broader function for GIT1 and GIT2/PKL as regulators of migrationrelated processes, including a...

The 68,000, 145,000, and 200,000 dalton subunits of neurofilaments from rat spinal cord and brain stem were shown by chemical phosphate analysis to contain about 3, 9, and 22 mol of phosphate/mol of polypeptide, respectively. All three subunits contained phosphoserine while the 145,000 dalton polypeptide also contained phosphothreonine. The high phosphate content of the 200,000 dalton polypepti...

Infectious bronchitis (IB) is an acute, highly contagious, and economically important viral disease of chickens. The S1 subunit from Spike (S) protein plays the major role in protective immunity and is involved in the host-virus interactions, as well as infectious bronchitis virus (IBV) serotyping. Aim of the present study was multi-aspect analysis of the molecular and immunological features of...

sites mapped by mass spectrometry Boris Ratnikov1,*,‡, Celeste Ptak2,*, Jaewon Han1, Jeffrey Shabanowitz2,3, Donald F. Hunt2,3 and Mark H. Ginsberg1 1Department of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 920930726 USA 2Department of Chemistry, University of Virginia, Charlottesville, VA 22908, USA 3Department of Pathology, University of Virginia, Charlottes...

sites mapped by mass spectrometry Donna J. Webb1,*,‡,§, Melanie J. Schroeder2,‡, Cynthia J. Brame4, Leanna Whitmore1, Jeffrey Shabanowitz2, Donald F. Hunt2,3 and A. Rick Horwitz1 1Department of Cell Biology, 2Department of Chemistry, and 3Department of Pathology, University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA 4Department of Biology, Centenary College of Louis...

Polarity along the anteroposterior axis of the Drosophila embryo is established by the activity of maternal gene products deposited into the egg during oogenesis. These activities direct specialised domains of zygotic gene expression required for the determination of cell fate (St. Johnston and Nüsslein-Volhard, 1992). The anterior system, which is required for the formation of the head and the...

background: stress is a cause of male infertility. although sex hormones and sperm quality have been shown to be low in stress, sperm physiology and testicular functional proteins, such as phosphotyrosine proteins, have not been documented. objective: to investigate the acrosome status and alterations of testicular proteins involved in spermatogenesis and testosterone synthesis in chronic stres...

KinasePhos is a novel web server for computationally identifying catalytic kinase-specific phosphorylation sites. The known phosphorylation sites from public domain data sources are categorized by their annotated protein kinases. Based on the profile hidden Markov model, computational models are learned from the kinase-specific groups of the phosphorylation sites. After evaluating the learned m...