background and aims. traditional methods of diagnosing periodontal diseases have their limitations, which include inability to provide information about the recent activity of the disease and difficulty in diagnosing the disease at an early stage when clinical changes have not started. this has necessitated the use of biomarkers designed to overcome these short-comings. materials and methods. a...

background: cryptorchidism is a common malformation in neonates; surgery or medical treatments are applied during childhood. untreated cryptorchid testes are in the risk of intratubular germ cell neoplasia (igcn) and consequently invasive testicular tumors which could be shown by immunohistochemistry staining for placental like acid phosphatase (plap) marker. objective: we designed this study t...

alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. in the present study, alkaline phosphatase gene was inserted into the pet15b vector. the recombinant dna was then expressed using iptg as an inducer. the expression of this enzyme was optimized by changing expression conditions...

To evaluate phosphorus (P) availability and the role of microorganisms in P dynamics barley rhizosphere, we constructed a rhizobox using two arable Andosols under different fertilization management regimens cultivated (Hordeum vulgare L. cv. Minorimugi) for 5 weeks. The phosphatase-labile pool organic (Po) was assessed phosphatase-addition approach combination with chemical extraction Po from s...

Muscle extracts were subjected to fractionation with ethanol, chromatography on DEAE-cellulose, precipitation with (NH4)2SO4 and gel filtration on Sephadex G-200. These fractions were assayed for protein phosphatase activities by using the following seven phosphoprotein substrates: phosphorylase a, glycogen synthase b1, glycogen synthase b2, phosphorylase kinase (phosphorylated in either the al...

Many phosphorus release experiments have been conducted in the water level fluctuation zone (WLFZ). However, effects of rhizospheres on risk soil remain unclear. In this study, twenty-four samples were collected from WLFZ Pengxi River, largest tributary northern Three Gorges Reservoir. Physicochemical properties, forms and phosphatase activity rhizosphere near-rhizosphere soils investigated. Bo...