Benzimidazoles are important antitubulin agents used in veterinary medicine and plant disease control. Resistance is a practical problem correlated with single amino acid changes in beta-tubulin and is often linked to greater sensitivity to phenylcarbamates. This negative cross-resistance creates opportunities for durable antiresistance strategies. Attempts to understand the molecular basis of ...

Earlier studies on the effects of the herbicide isopropyl iV-phenylcarbamate (IPC) on mitosis revealed blocked metaphases, multinucleate cells, giant nuclei and an increase in number of partly contracted chromosomes. It was assumed that IPC, like colchicine, was causing these effects by disruption of the spindle apparatus by destroying the spindle microtubules. The animal hormone melatonin caus...

We describe enzymological and structural analyses of the interaction between the family 18 chitinase ChiB from Serratia marcescens and the designed inhibitor N,N'-diacetylchitobionoxime-N-phenylcarbamate (HM508). HM508 acts as a competitive inhibitor of this enzyme with a K(i) in the 50 microM range. Active site mutants of ChiB show K(i) values ranging from 1 to 200 microM, providing insight in...

esters with carbonyl, phosphoryl, and thionyl linkages, are subject to enzymatic hydrolysis. Pesticide hydrolysis by esterases and amidases from plants and microorganlsms can serve as a deloxification or activation mechanism that can govern pesticide sel€ctivity or resistance, and initiate or determine the rate of pesticide biodegradation in the environment. Substrate specificity of esterases a...

We have previously shown that preischemic treatment with glucosamine improved cardiac functional recovery following ischemia-reperfusion, and this was mediated, at least in part, via enhanced flux through the hexosamine biosynthesis pathway and subsequently elevated O-linked N-acetylglucosamine (O-GlcNAc) protein levels. However, preischemic treatment is typically impractical in a clinical sett...

Modification of proteins at serine or threonine residues with N-acetylglucosamine, termed O-GlcNAcylation, plays an important role in most eukaryotic cells. To understand the molecular mechanism by which O-GlcNAcylation regulates the entry of Caenorhabditis elegans into the non-aging dauer state, we performed proteomic studies using two mutant strains: the O-GlcNAc transferase-deficient ogt-1(o...

Sensitivity to the two anti-microtubular drugs oryzalin and EPC (ethyl-N-phenylcarbamate) is shown to be uncoupled in the rice EPC-resistant ER31d cell line, derived from the corresponding ER31 mutant. The ER31d cell line grows in the presence of EPC but it remains susceptible to oryzalin. In the presence of concentrations of EPC up to 0.4 mM, ER31d cells remain viable maintaining cell anisotro...