In the present study, Phenylalanine ammonia lyase (PAL) activity was detected in different parts of grape plant (leaves, pulp, whole seeds and defatted seeds) extracts. The results revealed that seed possess highest PAL (69.44 U), followed by (44.22 whereas extracts leaves pulp gave lowest values (22.77 5.11 U, respectively). optimum conditions for crude extraction achieved using 50mM potassium...

Lignin is one of the major characteristics of plant secondary cell wall that provides structural rigidity for the cells and tissues and hydrophobicity to tracheary elements. Internode tissues of Aeluropus littoralis as a halophyte grass were sampled at different developmental stages (from the first to the fifth internodes ) and under different NaCl concentrations. The influences of NaCl and int...

BACKGROUND Some types of flavonoid intermediates seemed to be restricted to plants. Naringenin is a typical plant metabolite, that has never been reported to be produced in prokariotes. Naringenin is formed by the action of a chalcone synthase using as starter 4-coumaroyl-CoA, which in dicotyledonous plants derives from phenylalanine by the action of a phenylalanine ammonia lyase. RESULTS A c...

The sequence of a phenylalanine ammonia-lyase (PAL; EC: 4.3.1.24) of the thermophilic and radiotolerant bacterium Rubrobacter xylanophilus (RxPAL) was identified by screening the genomes of bacteria for members of the phenylalanine ammonia-lyase family. A synthetic gene encoding the RxPAL protein was cloned and overexpressed in Escherichia coli TOP 10 in a soluble form with an N-terminal His6-t...

Phenylalanine ammonia lyase (PAL) is the first enzyme in phenylpropanoid pathway and plays a critical role plant growth, development stress defence. However, there have been few reports of PAL gene family lucerne (also known as alfalfa, Medicago sativa L.), one most important forage legume species worldwide. In this study, we report that encoded by seven genes: MsPAL1–MsPAL7. Furthermore, compr...

Abiotic stress, like heat treatment and wounding, applied to pineapple by-products induce the accumulation of new compounds add value. In this work effect individual or combined application wounding stresses on total phenolic content, antioxidant activity through complementary methods (DPPH, FRAP, ABTS) enzymatic (bromelain, phenylalanine ammonia lyase (PAL) polyphenol oxidase) were evaluated. ...

So far only little data have been available concerning the eliciting capacity of well defined glycan molecules isolated from plant pathogens. This study brings new information about changes in plant cells caused by fungal pathogens. Sugar fractions derived from glycoproteins isolated from the fungus Fusarium sp. M7-1 have been tested here as signaling molecules. The ability of three O-glycan fr...

Given the essential role played by phenol metabolism in many resistance responses to different types of stress, the aim of the present work was to determine how different application rates of calcium may influence this metabolic process. Increased calcium in the nutrient solution in which tobacco plants were grown considerably reduced the foliar concentration of phenolic compounds. Calcium clea...

A cDNA clone for phenylalanine ammonia-lyase (PAL) induced in wounded sweet potato (Ipomoea batatas Lam.) root was obtained by immunoscreening a cDNA library. The protein produced in Escherichia coli cells containing the plasmid pPAL02 was indistinguishable from sweet potato PAL as judged by Ouchterlony double diffusion assays. The M(r) of its subunit was 77,000. The cells converted [(14)C]-l-p...