The interaction of the cysteine conjugate S-(1-chloro-1,2,2, -trifluoroethyl)-L-cysteine (CTFC) with organic anion and amino acid transport in the basolateral and apical membranes was examined with rabbit renal proximal tubule suspensions and primary cultures of rabbit renal proximal tubule cells. The apparent K(i) for CTFC inhibition of the 1-min uptake of [(3)H]p-aminohippurate in tubule susp...

Some mammals hibernate in response to harsh environments. Although hibernating mammals may metabolize proteins, the nitrogen metabolic pathways commonly activated during hibernation are not fully characterized. In contrast to the hypothesis of amino acid preservation, we found evidence of amino acid metabolism as three of five key enzymes, including phenylalanine hydroxylase (PAH), homogentisat...

In vitro adsorption of plasma proteins such as immunoglobulin (Ig)-G and IgM without the removal of albumin and fibrinogen forms the basis of the field of therapeutic immunoadsorption. In the present study, we investigated the activities of adsorbents with thienyl amino acids, tryptophan, or phenylalanine as ligands. IgG adsorption by an adsorbent with a thienyl amino acid ligand was lower than...

Stable isotope infusion methods have not been extensively used in horses to study protein metabolism. The objectives were to develop infusion and sampling methodologies for [1-(13)C] phenylalanine and apply these methods to determine whether the addition of supplemental amino acids to a control diet affected whole-body phenylalanine kinetics in mature horses. Arabian geldings were studied using...

objective(s) motility plays a major role in pathogenicity of helicobacter pylori, yet there is  scarce data regarding its chemotactic behaviour. the present study was designed to investigate the chemotactic responses of local isolates of h. pylori towards various sugars, amino acids, as well as some other chemical substances. materials and methods chemotaxis was assayed by a modified adler’s me...

Increasing amino acid availability (via infusion or ingestion) at rest or postexercise enhances amino acid transport into human skeletal muscle. It is unknown whether alterations in amino acid availability, from ingesting different dietary proteins, can enhance amino acid transport rates and amino acid transporter (AAT) mRNA expression. We hypothesized that the prolonged hyperaminoacidemia from...

Recently we reported the microbiological determination of L-phenylalanine and Land n-phenyllactic acid with Lactobacdlus arabinosus as the test organism (1). Although phenylalanine is not an absolute dietary essential to this organism, a microbiological determination of the amino acid is possible, for, in the absence of the amino acid or its metabolic derivatives, there is no visible growth at ...

BACKGROUND Phenylalanine, which is an essential aromatic amino acid, is either used for protein synthesis or irreversibly hydroxylated to tyrosine. The provision of optimal amounts of dietary phenylalanine is not only important for growth and development but might also influence catecholamine synthesis and release rates. The current recommended aromatic amino acid requirement for infants aged 0...

Although it is recognized that the abnormal accumulation of amino acid is a cause of the symptoms in metabolic disease such as phenylketonuria (PKU), the relationship between disease severity and serum amino acid levels is not well understood due to the lack of experimental model. Here, we present a novel in vitro cellular model using K562-D cells that proliferate slowly in the presence of exce...