A phenanthrene degrader, Mycobacterium sp. EPa45, was isolated from a phenanthrene-degrading consortium. Here, we report the complete genome sequence of EPa45, which has a 6.2-Mb single circular chromosome. We propose a phenanthrene degradation pathway in EPa45 based on the complete genome sequence.

Introduction: Pyrene and phenanthrene are polycyclic aromatic hydrocarbons (PAHs) are priority pollutants. The aim of this study was to investigate the ability of isolated bacteria from stabilized compost‌ for biodegradation of pyrene and phenanthrene from soil contaminated with municipal solid waste leachate. Materials and Methods: In this study, phenanthrene and pyrene were selected as prior...

Three strains of Comamonas testosteroni were isolated from river sediment for the ability to degrade phenanthrene; two of the strains also grew on naphthalene, and one strain also grew on anthracene. The homology of the genes for polycyclic aromatic hydrocarbon degradation in these strains to the classical genes (nah) for naphthalene degradation from Pseudomonas putida NCIB 9816-4 was determine...

We investigated bioavailability, as measured by the biota-sediment accumulation factor (BSAF), of reversibly sorbed and desorption-resistant phenanthrene to the deposit-feeding freshwater tubificid oligochaete Ilyodrilus templetoni. Desorption-resistant, phenanthrene-contaminated sediments were prepared by a sequential batch desorption method by washing with an isopropanol solution. The BSAFs a...

Bacteria that are capable of degrading polycyclic aromatic hydrocarbons were enumerated by incorporating soil and water dilutions together with fine particles of phenanthrene, a polycyclic aromatic hydrocarbon, into an agarose overlayer and pouring the mixture over a mineral salts underlayer. The phenanthrene-degrading bacteria embedded in the overlayer were recognized by a halo of clearing in ...

Exposure to polycyclic aromatic hydrocarbons (PAHs) in the food chain is the major human health hazard associated with the Deepwater Horizon oil spill. Phenanthrene is a representative PAH present in crude oil, and it undergoes biological transformation, photooxidation, and chemical oxidation to produce its signature oxygenated derivative, phenanthrene-9,10-quinone. We report the downstream met...

The mutagenic activity of benzo(c)phenanthrene and some of its known and potential metabolites was evaluated in bac terial and mammalian cells either in the presence or absence of a metabolic activation system. trans-3,4-Dihydroxy-3,4-di hydrobenzo(c)phenanthrene [benzo(c)phenanthrene 3,4-di hydrodiol] was metabolized by a cytochrome P-450-dependent monooxygenase system to products which were s...