The colloidal 2D materials based on graphene and its modifications are of great interest when it comes to forming LC phases. These phases allow controlling the orientational order particles, paving way for efficient processing modified with anisotropic properties. Here, we present peculiarities AA functionalization GO, along formation phase behavior in an external magnetic field. We discuss inf...

Staphylococcal enterotoxin C (SEC) is a major cause of staphylococcal food poisoning in humans and plays role bovine mastitis. Staphylococcus aureus (S. aureus) benefits from competitive growth advantage under stress conditions encountered foods such as low pH. Therefore, understanding the stressors lactic acid on SEC production pivotal relevance to safety. However, stress-dependent cues their ...

We report on advancements of our microscale isoelectric fractionation (IEFr) methodology for fast on-chip separation and concentration of proteins based on their isoelectric points (pI). We establish that proteins can be fractionated depending on posttranslational modifications into different pH specific bins, from where they can be efficiently transferred to downstream membranes for additiona...

Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...

3-Aminophenyl-modified analogues of the bicyclic nucleoside N-methyl-3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one were synthesised and incorporated directly into triplex-forming oligonucleotides in order to utilise their extended hydrogen bonding motif for recognition of the CG base pair. All analogues demonstrated strong binding affinity and very good selectivity for CG from pH 6.2 to 7.0; a marked im...

Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...

Single cell gel electrophoresis (SCGE) or comet assay is a rapid and sensitive fluorescent microscopic method which allows measurement of DNA strand breaks in individual cells. Modifications of SCGE conditions permitted to detect different types of DNA damage. In order to characterize DNA damage induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and hydrogen peroxide (H2O2) in Chinese hamst...

The effect of pH on the Qy absorption band was studied in the isolated D1-D2-cytochrome b559 complex. The pH-treatments were done on an ionexchange chromatographic column. The absorption spectra at 77K of the complex treated with acidic pH showed irreversible loss of absorbance at both the blue and the red sides of the Qy absorption band with minima at 664.5 and 683.5 nm, respectively. These ab...

General aspects of modeling as an engineering tool are presented in this work. The usefulness of Takagi-Sugeno Fuzzy Inference Systems (TS FIS) as modeling technique are emphasized. Additionally, various modifications of known methodology for identifying such a models and rules configuration are proposed. Finally, two application examples are presented. The first one is a modeling of the Box an...