Background: Abortion is the interruption and/or termination of pregnancy, either spontaneously or intentionally, before fetus develops sufficiently to survive. About 80% abortions occur in first 12 weeks pregnancy. The underlying mechanism about half cases involves chromosomal abnormalities. Objective: To evaluate association between TSH, T3, T4, anti-thyroglobulin antibody and thyroid peroxida...

Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the possibility to tailor their mobility gives a huge potential for diagnostic assays. An enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase (HRP) as label, proved integr...

An improved enzyme-linked immunosorbent assay (ELISA) for detection of heterophile Hanganutziu-Deicher (HD) antibodies and antigens, which are frequently detected in sera and/or cancerous tissues from patients with various cancers was developed using biotinylated chicken anti-GM3(NeuGc) antibody and avidin-horseradish peroxidase conjugate. The N-glycolylneuraminyllactosyl-ceramide, GM3(NeuGc) g...

Antibody to tissue factor apoprotein was adsorbed against gamma-globulin and coupled to horseradish peroxidase; this complex was applied to various rabbit tissues. The distribution of the peroxidase marker then was observed by electron microscopy. We examined fixed or frozen sections, as well as breis of aorta, vena cava, brain, heart, lung, liver, spleen, kidney, bone marrow, mesothelial gut l...

The phenotype of fresh and cultured leukemic cells from patients with hairy cell leukemia was studied using a panel of monoclonal antibodies in addition to the detection of peroxidase activity under electron microscopy. In fresh samples, the leukemic cells from 11 patients displayed predominantly a B phenotype, as judged by their reactivity with the B1 monoclonal antibody and surface immunoglob...

We describe an internally referenced immunochemical test-strip for use in the rapid detection of morphine. The method is based on the "enzyme-channelling" immunoassay technique, and a glucose oxidase-horseradish peroxidase enzyme pair is used to immunospecifically generate an insoluble, colored reaction product on the test-strip surface. Test strips are composed of two active surfaces, each of ...

OBJECTIVES To evaluate the role of thyroid ultrasound in children with autoimmune thyroiditis diagnosed either on cytopathology or by the presence of thyroid peroxidase antibodies. DESIGN AND SETTINGS Children presenting for the first time to the Thyroid Clinic at the Institute of Nuclear Medicine and Allied Sciences for the complaint of goiter over a two year period (January 2005-December 20...

when duplicate blots were probed with the avidin-peroxidase conjugate (Figure 1B, lanes 5′–8′) ExtrAvidin® (Sigma, St. Louis, MO, USA). Unlike the secondary antibody conjugate used in Figure 1A, the avidin-peroxidase conjugate used in Figure 1B did not recognize the electrophoresed immunoglobulins within the immunoprecipitate. Moreover, specific bands of ezrin were readily detected in immunopre...