We have found that natural killer (NK) cells were very active in pleural effusions containing Mycobacterium tuberculosis. Cytotoxicity against K562 and Raji was augmented when the mononuclear cells were cocultured for 18 hr with purified protein derivative (PPD) derived from M. tuberculosis culture supernatants. In pleural effusions of cancer patients, PPD-activated mononuclear cells were less ...

introduction: enrichment of intact peripheral blood dendritic cells (dcs) has been done using rosette and immunomagnetic depletion techniques. material and methods: peripheral blood mononuclear cells were separated using ficoll density gradient centrifugation and t lymphocytes were depleted through reaction with aet- treated sheep red blood cells. the remaining cells were depleted from lineage ...

Background: Several biological and medical benefits of Saffron, Crocus sativus (Iridaceae), have been demonstrated. However, mechanisms of actions for purified constituents are greatly unknown. Objective: To examine the effects of Safranal, a main constituent of Saffron stigma, on cell viability and cytokine profile of peripheral blood mononuclear cells (PBMC) were examined. Methods: Effect...

Background:The aim of this study was to evaluate the effect of Ramadan fasting on SIRT1 mRNA expression in healthy men.Islamic Ramadan fasting is a holy religious ceremony that has many spiritual benefits. Additionally, it can be considered as the equivalent of calorie restriction that may affect physical health. The results of previous studies revealed that calorie restriction increases the li...

Objective: To explore the expression and clinical significance of miR-155 miR-146a in psoriasis patients from different sources. Methods: The levels vulgaris tissues, peripheral blood mononuclear cells, serum were detected by QRT-PCR method 30 with healthy subjects as normal control group, correlation between samples sources was analyzed. And after treatment. Results: skin tissue, cells signifi...