We developed mouse monoclonal antibodies (Abs) against pepsinogen C with highly purified antigen isolated from gastric mucosa. The Abs were used to construct a two-site sandwich-type assay for pepsinogen C with time-resolved fluorometry as a detection technique. The assay has a detection limit of 0.1 microgram/L and is precise (within-run and day-to-day CVs < 11%). We used this assay to measure...

Pepsinogen, the precursors of pepsin, is classified into two subtypes: pepsinogen I (PG I) and pepsinogen II (PG II). Patients with impaired renal function are associated with elevated concentrations of serum pepsinogen. Contradictory results have been reported about the effect of dialysis on the serum pepsinogen levels, as the previous studies were conducted only in a particular period of dial...

OBJECTIVE To determine whether there is an association between otitis media with effusion and laryngopharyngeal reflux in children. MATERIALS AND METHODS This study included 31 children with otitis media with effusion. The pepsinogen level in the middle ear fluid of all patients was measured by sandwich enzyme-linked immunosorbent assay. Each patient's middle ear fluid was investigated for He...

Serum pepsinogen activity was determined in the blood serum of 126 clinically healthy cows by the method of Paynter. After slaughter the cows studied were classified into three groups according to established macroscopic changes to the mucous membrane of the abomasum: a group without changes, a group with ulcers and a group with other changes. The prevalence of the appearance of abomasal ulcers...

A system for the production of soluble porcine pepsinogen A (EC 3.4.23.1) was developed by fusing the pepsinogen and thioredoxin genes and then expressing the fused product (Trx-PG) in Escherichia coli. The expressed fusion protein was purified using a combination of ion-exchange and hydrophobic chromatography. Trypsin digestion of the fusion protein yielded pepsinogen which was one residue lon...

The pepsinogens of human gastric mucosa have been the subject of several studies using electrophoretic and chromatographic techniques (Uriel, 1960; Seijffers, Segal, and Miller, 1963; Tang and Tang, 1963; Kushner, Rapp, and Burtin, 1964; Rapp, Aronson, Burtin, and Grabar, 1964; Hanley, Boyer, and Naughton, 1966; Taylor, 1968; Samloff, 1969); it has been shown that gel electrophoresis is superio...

Both carbachol (10(-4)-10(-3) mol/l) and cholecystokinin octapeptide (CCK-8) (10(-8)-10(-6) mol/l) significantly stimulated the release of pepsinogen from rabbit gastric mucosa maintained in organ culture (213-216% and 143-261% of control, respectively, p less than 0.05-0.01). The secretion was not affected by removing Ca2+ from the culture medium with ethylene glycol tetra-acetic acid. Verapam...

The relation between Helicobacter pylori (H pylori) infection and fasting gastrin and pepsinogen-I and -II concentrations was evaluated in 278 volunteers without symptoms and the results were compared with the values obtained in 35 patients with duodenal ulcers. H pylori infection was determined with the 13C-urea breath test in subjects without symptoms and with endoscopy, biopsy (histology and...