Pepsin has been employed successfully in conjunction with other proteolytic enzymes for the degradation and structural determination of insulin (1, 2), corticotropin (3), ribonuclease (4), and tobacco mosaic virus (5). Because of the difference in specificity between this enzyme and chymotrypsin (6), it was thought that pepsin would hydrolyze some bonds not attacked by chymotrypsin and perhaps ...

The study reports a case of "pure" tomato allergy in an adult female. The responsible allergen was partially characterized by immunoblot analysis, pepsin digestion, and heating. It had a molecular weight of about 9 kDa and was heat-labile and pepsin-resistant, thus confirming the clinical history. Unfortunately, due to the failure of both 2-dimensional electrophoresis analysis and N-terminal se...

The effects have been examined of chymotrypsin, pepsin, trypsin, and pancreatic lipase on cattle rhodopsin in digitonin solution. The digestion of rhodopsin by chymotrypsin was measured by the hydrolysis of peptide bonds (formol titration), changes in pH, and bleaching. The digestion proceeds in two stages: an initial rapid hydrolysis which exposes about 30 amino groups per molecule, without bl...

The undifferentiated F9 embryonal carcinoma cells produce a unique collagen that decreases in amount during retinoic acid-induced differentiation of F9 cells into basement-membrane parietal endoderm. A bacterial-collagenase-sensitive protein of approx. 60,000 Da was resolved on polyacrylamide-gel electrophoresis. After pepsin digestion, two pepsin-resistant fragments containing hydroxyproline w...

IN a previous paper [Clifford, 1925] the effects of the halogen salts of sodium, potassium, ammonium, and calcium on salivary digestion were described. These salts were found to vary in their action on ptyalin, some delaying digestion, others being inert, and some increasing the speed of reaching the achromic point. It was thought advisable to continue the investigation with other enzymes, and ...

In this study, an apricot kernel milk suspension was prepared, for which Hac?halilo?lu-type raw and roasted kernels were used. Protein, total lipid, ash contents of determined. Palmitic acid, oleic linoleic acid considered as major fatty acids according to gas chromatography. During in vitro gastrointestinal digestion, the proteins hydrolyzed pepsin-resistant No difference recorded between panc...

In the present study, in vitro digestibility and structure of soybean protein isolates (SPIs) prepared from five soybean varieties were investigated in simulated gastric fluid (SGF), using FT-IR microspectroscopy and SDS-PAGE. The result indicated that β-conformations were prone to be hydrolyzed by pepsin preferentially and transformed to unordered structure during in vitro digestion, followed ...

When milk is ingested, casein micelles will be successively digested by pepsin in the stomach and trypsin in the intestine. Therefore, we digested casein micelles successively with pepsin at pH 4.0 and trypsin at pH 7.0, and recovered casein phosphopeptides (CPP) as CPP-calcium phosphate (CP) complexes. The CPP-CP complexes contained 248 mg of calcium/g peptides and 175 mg of inorganic phosphor...

Human serum antibodies which react different pepsin-digested IgA proteins only with antigenic determinants rewas found in individual sera but no revealed by digestion of IgA with pepsin lationship to either IgA subgroups or were found in 29.2 per cent of the sera genetically determined IgA antigens was from 369 hospitalized patients studied. discerned. The presence of these antiThese antibodies...

BACKGROUND AND OBJECTIVES Recently, type II collagen (CII) was found to be effective clinically for treatment of rheumatoid arthritis (RA). However, the molecular properties of CII could be changed during the preparation process. In the present study, we isolated CII from chick sternal cartilage and studied the structural characteristics of purified CII. MATERIALS AND METHODS Pepsin-solubiliz...