The plant-pathogenic bacterium Dickeya dadantii produces several pectinolytic enzymes that play a major role in the soft-rot disease. Eight characterized endopectate lyases are secreted in the extracellular medium by the type II secretion system, Out. They cleave internal glycosidic bonds of pectin, leading to plant tissue maceration. The D. dadantii pectate lyases belong to different families,...

Pectin lyase and polygalacturonase production by newly isolated Penicillium viridicatum strain Rfc3 was carried out by means of solid state fermentation using orange bagasse, corn tegument, wheat bran and mango and banana peels as carbon sources. The maximal activity value of polygalacturonase (Pg) (30U.g) was obtained using wheat bran as carbon source while maximal pectin lyase (Pl) (2000 U.g)...

Abstract Pectin from two different plant sources: apple and sugar beet was studied. Apple pectinwas modified by chemical treatments such as: acid, alkali and ammonia, so as to obtain an ester levelequal to or less than 50 %, which is described as low methoxyl (LM) pectin. LM pectin form gelswith significant characteristic. In this study, de-esterification of apple pectin with acid treatmentresu...

Production of enzymes degrading plant cell walls was studied using media containing cellobiose or ammonium ions (NH,+) as limiting nutrients. Carboxymethylcellulase (CMcellulase), xylanase and pectin lyase were primarily cell-associated during exponential growth in batch culture but accumulated in the supernatant during the stationary phase. Activities of CM-cellulase and xylanase were higher i...

Special features of the protopectin complex structure plant tissue suggest necessity performing point destruction certain glycoside bonds in rhamnogalacturonan polymer chains for industrial production pectin. These include homogalacturonan sites and branching zones. As fragments carry main functional load, between residues rhamnose galacturonic acid are targeted bonds. For their directional des...

Pectinophiles are bacteria that utilize pectin and only a few related compounds as substrates. Obligately anaerobic pectinophiles have been isolated from the intestinal tracts and gingivae of humans and from the rumina of cattle. We isolated three strains of pectinophilic bacteria from colonic contents of pigs but were unable to isolate pectinophiles from the rumen contents of four sheep, even ...

Verticillium wilt, caused by the Verticillium dahliae phytopathogen, is a devastating disease affecting many economically important crops. Previous studies have shown that the exoproteome of V. dahliae plays a significant role in this pathogenic process, but the components and mechanisms that underlie this remain unclear. In this study, the exoproteome of V. dahliae was induced in a cotton-cont...

The characteristics of the pectic enzyme polygalacturonase (PG) of Phytophthora meadii isolates from petioles of rubber (Hevea brasiliensis) genotypes were compared in relation to (i) aggressiveness (virulence) in infecting detached petioles of a susceptible rubber genotype (PB86) or (ii) in relation to the availability of purified rubber petiole cell walls (obtained from a Phytophthora-resista...

Fruit ripening and softening indicated by firmness determines the texture transportability, shelf life of tomato products. However, regulatory mechanism underlying formation in is different varieties overall fruit poorly understood. Therefore, this study, physical, biochemical, molecular properties three varieties; ‘Sarikiz’ (yellow skin colour), ‘Moda’ (orange colour) ‘Red Type Cherry’ (red at...