Relative molecular size distributions of pectic and hemicellulosic polysaccharides of pea (Pisum sativum cv Alaska) third internode primary walls were determined by gel filtration chromatography. Pectic polyuronides have a peak molecular mass of about 1100 kilodaltons, relative to dextran standards. This peak may be partly an aggregate of smaller molecular units, because demonstrable aggregatio...

Concentrated binary mixtures of polymers in solution commonly exhibit immiscibility, resolving into two separate phases each of which is enriched in one polymer. The plant cell wall is a concentrated polymer assembly, and phase separation of the constituent polymers could make an important contribution to its structural organization and functional properties. However, to our knowledge, there ha...

The genus Fusarium is a species rich genus. Different media are required to study the morphological characters and classify different species, a time consuming technique. In this study pectic zymograms were used to identify species and form species of Fusarium isolates. Fusarium isolates (318) were obtained from different areas and hosts in Isfahan Province, Iran. Isolates were identified to sp...

The natural pectins are believed to consist of long, open, unbranched chains of galacturonic acid with an occasional arabinose and galactose molecule. The free carboxyl groups of the galacturonic acid are partially or wholly esterified with methyl alcohol, and an occasional hydroxyl group is esterified with acetic acid [BONNER (4), BRANFOOT (5), FREUDENBERG (7), GADDUM (8), KERTESZ (13), MYERS ...

rhe cuiticle of plants is a heterogenous memnbranie in which wax, pectin and cellulose in varying l)ro)ortions are contained in a cutin franmework. In the isolation of cuticular membranes from leaves or fruits the wax is nornially removed by extraction xvith ani organic solvent before detachment of the memnbranes from the underlying epidermal cells. Detachnmenit is achieve(d by the use of eithe...

Pectins are a group of plant polysaccharides whose principal component is 1,4-linked a-d-galactosyluronic acid (O’Neill et al., 1990). Pectins are present in the primary cell walls of all seed-bearing plants and in the junction between cells called the middle lamella. The primary wall surrounds growing plant cells, meristematic cells and cells in succulent tissue such as fruit. The secondary wa...

Yeast polygalacturonase (YPG) catalyzes the hydrolysis of pectic acid to a mixture of digalacturonic and galacturonic acids (1). The reaction course consists of (a) an initial rapid attack on the linear chain by a random mechanism until about 25 per cent hydrolysis has occurred, (b) a slower phase during which an additional 25 per cent of the available bonds are broken, and (c) an extremely slo...