A central step in vertebrate visual transduction is the rapid drop in cGMP levels that causes cGMP-gated ion channels in the photoreceptor cell membrane to close. It has long been a puzzle that the cGMP phosphodiesterase (PDE) whose activation causes this decrease contains not only catalytic sites for cGMP hydrolysis but also noncatalytic cGMP binding sites. Recent work has shown that occupancy...

In 32PO4-labeled adipocytes, isoproterenol (ISO) or physiologically relevant concentrations of insulin rapidly increased phosphorylation of a particulate 135-kDa protein which has been identified as a cGMP-inhibited "low Km" cAMP phosphodiesterase (CGI-PDE) by several criteria, including selective immunoprecipitation with anti-CGI-PDE IgG (Degerman, E., Smith, C.J., Tornqvist, H., Vasta, V., Be...

PDE surfaces, whose behavior is governed by Partial Differential Equations (PDEs), have demonstrated many modeling advantages in surface blending, free-form surface modeling, and surface’s aesthetic or functional specifications. Although PDE surfaces can potentially unify geometric attributes and functional constraints for surface design, current PDE-based techniques exhibit certain difficultie...

Partial differential equation (PDE) models are commonly used to model complex dynamic systems in applied sciences such as biology and finance. The forms of these PDE models are usually proposed by experts based on their prior knowledge and understanding of the dynamic system. Parameters in PDE models often have interesting scientific interpretations, but their values are often unknown, and need...

While the pathogenesis of chronic obstructive pulmonary disease (COPD) is incompletely understood, chronic inflammation is a major factor. In fact, the inflammatory response is abnormal, with CD8+ T-cells, CD68+ macrophages, and neutrophils predominating in the conducting airways, lung parenchyma, and pulmonary vasculature. Elevated levels of the second messenger cAMP can inhibit some inflammat...

Nitric oxide (NO) functions as a diffusible messenger in the central nervous system and elsewhere, exerting many of it physiological effects by activating soluble guanylyl cyclase, so increasing cellular cGMP levels. Hydrolysis of cyclic nucleotides is achieved by phosphodiesterases (PDEs) but the enzyme isoforms responsible for degrading cGMP in most cells have not been identified. We have dev...

PURPOSE Phosphodiesterase (PDE) inhibitors are important therapeutic agents, but their effects on photoreceptor PDE (PDE6) and photoreceptor cells are poorly understood. The potency and selectivity of various classes of PDE inhibitors on purified rod and cone PDE6 and on intact rod outer segments (ROS) were characterized. METHODS The inhibition constant (K(i)) of isozyme-selective PDE inhibit...

Although expression of natriuretic peptides in cardiac tissues is up-regulated in response to pressure overload, no significant change in cGMP level in hypertrophied ventricles was observed. Activities of two cyclic nucleotide phosphodiesterase (PDE) isoforms, Ca2+/calmodulin-stimulated PDE (PDE1) and cGMP-stimulated PDE (PDE2), were significantly higher in rat left ventricles 14 days after aor...

An experimental study on pulse detonation engine (PDE) is conducted using hydrogen/air mixtures. Several key issues for PDE development, including valve operation, injection, mixing, filling, cycle repetition, ignition timing, DDT distance and propagation of detonation/quasi-detonation, are investigated. The fuel and oxidizer are injected into PDE from opposite sidewall directions to be well mi...

The population density equation (PDE) has generated considerable interest, recently. The PDE allows e.cient simulation of populations with a large number of neurons. We apply the PDE to an existing model of delay activity, which produces stationary 1ring rates for delay assemblies. In order to observe transient phenomena, we apply the PDE to this model. Here we present the preliminary results. ...