BACKGROUND Salmonellosis is of great economic concern in all phases of the poultry industry, from production to marketing, leading to severe economic losses. Monitoring the source of the bacterial contamination has fundamental importance in the spreading of salmonellosis. RESULTS We applied a ligation-mediated PCR method, PCR MP (PCR melting profile), to type S. enterica ssp. enterica ser. En...

With the objective of identifying presence Paratuberculosis (PTB), an infectious disease caused by Mycobacterium avium ssp paratuberculosis (MAP), in sheep, through pathological studies, bacterial culture and IS900 PCR, as well estimating seroprevalence to MAP. The present cross-sectional study, was conducted 16 different flocks, with serum 2415 adult sheeps, analyzed Enzyme-Linked ImmunoSorben...

  Background and Objective: The outcome of hepatitis B virus (HBV) infection may be influenced by host factors like Human Leukocyte Antigen (HLA). We have investigated HLA-A and DRB1 alleles in patients with persistent hepatitis B infection compared to subjects who had spontaneously recovered from HBV infection. To complete the findings of this study we performed another survey in certain HLA ...

Major histocompatibility complex (MHC) class II DRB genotypes were examined in two geographically isolated populations of California sea lions (Zalophus californianus) (Gulf of California and California coastal Pacific Ocean). Genomic DNA from 227 California sea lions was examined using eight sequence-specific primer (SSP) pairs flanking the putative peptide-binding site. A total of 40 differen...

and Granulocyte Immunobiology, S’Agaró, Girona, Spain, May 9-12, 1998, p 52 (abstr) 6. Hessner MJ, Curtis BR, Endean DJ, Aster RH: Determination of neutrophil antigen NA gene frequencies in five different ethnic groups by the polymerase chain reaction with sequence-specific primers (PCR-SSP). Transfusion 36:895, 1996 7. Lin M, Chen CC, Wang CL, Lee HL: Frequencies of neutrophilspecific antigens...

The genus Montenegrina is revised on the basis of material available at the Hungarian Natural History Museum (Budapest), Naturhistorisches Museum Wien (Vienna), and the Naturmuseum Senckenberg (Frankfurt am Main), as well as newly discovered populations. The following new taxa are described: Montenegrina haringae sp. n., Montenegrina lillae sp. n., Montenegrina prokletiana sp. n., Montenegrina ...

and Granulocyte Immunobiology, S’Agaró, Girona, Spain, May 9-12, 1998, p 52 (abstr) 6. Hessner MJ, Curtis BR, Endean DJ, Aster RH: Determination of neutrophil antigen NA gene frequencies in five different ethnic groups by the polymerase chain reaction with sequence-specific primers (PCR-SSP). Transfusion 36:895, 1996 7. Lin M, Chen CC, Wang CL, Lee HL: Frequencies of neutrophilspecific antigens...

and Granulocyte Immunobiology, S’Agaró, Girona, Spain, May 9-12, 1998, p 52 (abstr) 6. Hessner MJ, Curtis BR, Endean DJ, Aster RH: Determination of neutrophil antigen NA gene frequencies in five different ethnic groups by the polymerase chain reaction with sequence-specific primers (PCR-SSP). Transfusion 36:895, 1996 7. Lin M, Chen CC, Wang CL, Lee HL: Frequencies of neutrophilspecific antigens...

OBJECTIVE To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories. METHODS The tests are based on PCR or NASBA amplification of the parasites nucleic acids followed by rapid read-out by oligochromatographic dipstick (PCR-OC and NASBA-O...