Resistance to multiple races of Fusarium wilt is a desired trait in lettuce (Lactuca sativa L.) cultivars, as it directly related grower profitability. We focused on its resistance the race 2 pathogen, and analyzed F2 individuals obtained from cross between ‘VI185’ (resistance 2) ‘ShinanoGreen’ (susceptible for genetic mapping locus. According ddRAD-seq analysis genotype prediction each individ...

the genotypes of the melatonin receptor 1a (mtnr1a) were determined by pcr-rflp in the native iranian shall  and karakul breeds of sheep . blood samples were collected from 60 karakul and 50 shall breeds. genomic dna was extracted based on the guanidin thiocyanate-slica gel method. after pcr reaction, pcr products were digested by the mnl1 restriction enzyme. the mtnr1a locus had two genotypes ...

Sweet potato is a hexaploid heterozygote with complex genetic background, self-pollination infertility, and cross incompatibility, which makes linkage analysis quite difficult. Genome-wide association studies (GWAS) provide new strategy for gene mapping cloning in sweet potato. Storage root flesh color (SRFC) an important sensory evaluation, correlates storage composition, such as starch, antho...

in order to develop molecular markers based on expressed sequence tag (est) linked to fusarium head blight (fhb) resistance genes in wheat, the biological function of 8660 ests deposited in data bank were studied following which 260 ests with identified biological function were selected. in total, 62 primer combinations were designed based on conserved and non conserved sequences. linkage and q...

Bacterial halo blight (BHB) is a bacterial disease, caused by Pseudomonas syringae pv. garcae, which has been gaining prominence in the main coffee-producing regions. Chemical control of this disease increases production costs and environmentally undesirable. In scenario, development new cultivars resistant to BHB most economical sustainable alternative. Marker-Assisted Selection (MAS) an appro...

The preservation of banana fruit is vital to ensure ripening quality during the transportation process. can be defined using gene expression marker, which needs high-quality RNA. However, when access laboratory difficult, long-distance transportation, lack liquid nitrogen or refrigerator, we need an alternative for methods, so sample and extracted RNA are still in excellent conditions. Aim our ...