نتایج جستجو برای: pcr and nested

تعداد نتایج: 16854787  

Journal: :archives of razi institute 2016
n. motamedi r. salehi tabar a.h. dalimi h. paykari s.a. ghorashi

trematodes are important in economic and public health. ornithobilharzia turkestanicum (o. turkestanicum) is one of the important economic trematodes in domestic animals. ornithobilharzia infection in intermediate host (lymnaea gedrosiana ) can be detected by either exposing snails to light to induce cercarial shedding or by squeezing them between glass slides to detect parasites. the current a...

Journal: :iranian journal of parasitology 0
aa eskandarian department of parasitology & mycology, faculty of medicine, esfahan university of medical sciences, esfahan, iran.

background: diagnosis of some diseases is difficult due to invasive sampling. urine has been candi-date as a non-invasive and convenient alternative. it has many advantages and easy accessibility but some technical ills should be removed. finding a suitable extraction method for improving urine dna quantity and quality in altering invasive specimens for molecular diagnosis of some infectious di...

Journal: :The Southeast Asian journal of tropical medicine and public health 2010
Orawan Ottiwet Chariya Chomvarin Kunyaluk Chaicumpar Wises Namwat Pisaln Mairiang

Sensitivity and specificity are important for tests used to defect Helicobacter pylori infection from gastric biopsy specimens. Molecular methods, such as PCR and nested PCR, are sensitive methods for H. pylori detection. The objective of this study was to evaluate the performance of PCR and nested PCR compared to culture, the rapid urease test (RUT) and histology for the diagnosis of H. pylori...

Journal: :medical journal of islamic republic of iran 0
tahereh shokohi from the department of parasitology, mycology and entomology. mazandaran university of medical sciences, sari, islamic republic of iran julie c. silver the division of life sciences, department of microbiology and molecular genetics, scarborough campus. university of toronto, ontario. canada

invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus s...

Background: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. Objectives: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR.   Materials and Methods: Genomic DNA from Bacillus thermo...

Journal: :jundishapur journal of microbiology 0
mahin jamshidi makiani antimicrobial resistance research center, iran university of medical sciences, tehran, ir iran parivash davoodian infectious disease research center, hormozgan university of medical sciences, bandar abbas, ir iran mahnaz baghershiroodi cellular & molecular research center, hormozgan university of medical sciences, bandar abbas, ir iran abdol azim nejatizadeh infectious disease research center, hormozgan university of medical sciences, bandar abbas, ir iran; cellular & molecular research center, hormozgan university of medical sciences, bandar abbas, ir iran farideh fakkhar bandar abbas health center, tb control center, hormozagan university of medical sciences, bandar abbas, ir iran mehrangiz zangeneh department of infectious disease, tehran medical branch, islamic azad university, tehran, ir iran; department of endocrinology and female infertility, reproductive biomedicine research center, royan institute for reproductive biomedicine, acecr, tehran, ir iran; department of infectious disease, tehran medical branch, islamic azad university, tehran, ir iran. tel: +98-2122618550, fax: +98-2155346301

background while tuberculosis (tb) can be diagnosed by microscopy and culture, the sensitivity of ziehl-neelsen staining is variable and culture results require 4 - 8 weeks to be determined. polymerase chain reaction (pcr) and its modifications, including nested pcr, might be promising methods for the rapid diagnosis of tb. objectives this study aimed to evaluate the performance of nested pcr o...

2015
Meysam Sharifdini Hossein Mirhendi Keyhan Ashrafi Mostafa Hosseini Mehdi Mohebali Hossein Khodadadi Eshrat Beigom Kia

This study was performed to evaluate nested polymerase chain reaction (PCR) and real-time PCR methods for detection of Strongyloides stercoralis in fecal samples compared with parasitological methods. A total of 466 stool samples were examined by conventional parasitological methods (formalin ether concentration [FEC] and agar plate culture [APC]). DNA was extracted using an in-house method, an...

Journal: :journal of agricultural science and technology 2013
s. jamali z. banihashemi

roots of helianthemum species were collected from various rangeland sites in fars, and other provinces in iran. the partial small subunits of ribosomal dna genes were amplified with the genomic dna extracted from their roots by nested polymerase chain reaction (pcr) using the universal fungal primer pair its1/its4 and specific primer pair ftc/rtc, which was designed based on internal transcribe...

Journal: :گوارش 0
reza ghanbari mehrdad ravanshad seyedunes hosseini kiana shahzamani

background: hepatitis g virus (gbv-c) is a single strand rnapositive virus and is a member of flaviviridae family. infection with the virus is prevalent add has worldwide distribution. accurate diagnosis of the virus is very important, because of co-infection with other important viruses like hcv, hbv and hiv, it may influence on pathogenesis, disease progression and response to viral therapy. ...

A Hovanesian, Mahdi Assmar, N. Nikolaova, Nasser Khodai Booran, Valery V. Bakayev,

Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for diagnosing infectious disease in the transplant recipients. We used this method for detection of Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested pr...

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