Background and objectives: Cutaneous leishmaniasis is endemic in most areas of Iran, and the diagnosis of its species is essential for controlling the disease. Leishmania major is the causative agent for cutaneous leishmaniasis in humans. Molecular methods are generally more sensitive than microscopic methods. The present study aimed to use a polymerase chain reaction-enzyme linked immunosorben...

Abstract Background Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at Xishuangbanna Blood Center, Yunnan, to support development strategies. Methods samples were collected randomly between June 2019 August 2019. These ...

Aims: In this study, it was aimed to investigate the occurrence of cucurbit yellow stunting disorder virus (CYSDV), chlorotic yellows (CCYV) and beet pseudo-yellows (BPYV) in cucumber, zucchini melon plants grown Hatay province by Double Antibody Sandwich-Enzyme Linked Immuno Sorbent Assay (DAS-ELISA) Reverse Transcription-Polymerase Chain Reaction (RT-PCR) methods, analyse phylogenetic relatio...

Toxoplasma gondii infects all species of warm-blooded animals, including humans, and domestic cats and other felidae are its definitive hosts. The aim of the present survey was to compare serological and molecular tests in accurate diagnosis of T. gondii infection among stray cats in Ahvaz, Iran. A total of 100 cats were examined for the detection of serum antibodies against T. gondii (chronic ...

BACKGROUND Foot-and-mouth disease (FMD) is an economically important and highly contagious viral disease that affects cloven-hoofed domestic and wild animals. Virus isolation and enzyme-linked immunosorbent assay (ELISA) are the gold standard tests for diagnosis of FMD. As these methods are time consuming, assays based on viral nucleic acid amplification have been developed. RESULTS A previou...

Objective: To perform comparative diagnostic study with rotavirus infected stool samples for efficacy ofindigenously prepared molecular transport medium (IMTM).Study Design: It was an experimental study.Place and Duration of Study: The conducted at Department Microbiology University Karachi,Karachi Pakistan from June to July 2021.Materials Methods: current based on hospital surveillance rotavir...

The present study assesses the efficacy of SVANOVIR Theileria annulata-Ab, the first commercial ELISA kit for the diagnosis of Theileria annulata infection in cattle based on a recombinant protein known as T. annulata surface protein (TaSp). As a reference test, a polymerase chain reaction (PCR) assay depending on T. annulata merozoite surface antigen (Tams-1) was applied. A total of 468 blood ...

A microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay (PCR-ELISA) has been used for the detection and identification of a variety of microorganisms. Here, we report the development of a PCR-ELISA for the identification of clinically relevant human rotavirus VP7 (G1 to G6, G8 to G10, and G12) and VP4 (P[4], P[6], P[8], P[9], and P[14]) genotypes. The G and P types of refer...

OBJECTIVES The objectives of this study were 1) to evaluate testing regimens of human immunodeficiency virus (HIV)-exposed infants and 2) to determine optimal methods of follow-up by enzyme-linked immunosorbent assay (ELISA) testing. METHODS We reviewed the results from 742 HIV-exposed infants in the state of North Carolina; 2474 samples were tested for HIV by DNA polymerase chain reaction (P...

Objective Chlamydia trachomatis (CT) infection is the most common sexually transmitted disease in the world that can persist and also ascend in the genital tract. This intracellular and silent infection is related to some adverse pregnancy outcomes, such as miscarriage. The aims of this study were to explore the best CT screening tests using blood and vaginal samples and to investigate the corr...