Source and Description. A partial genomic library (300 to 500 bp) was constructed using DNA obtained from Japanese flounder,Paralichthys olivaceus, digestedwith Sau3AI. The DNA was inserted in pBluescript SK (Toyobo, Japan) digested by BamHI and transformed into competent E. coli cells (strain DH5α). The partial genomic library was screened with a γP-labeled (CA)10 probe. About 800 positive clo...

Chemokines are important chemoattractant inflammatory molecules, but their interdependent network in disease pathogenesis remains unclear. Studies in mouse models have shown that herpetic stromal keratitis (SK) is produced by the consequence of a tissue-destructive immunoinflammatory reaction involving herpes simplex virus type 1 (HSV) infection. Here we found that ocular HSV infection leads to...

Let sk = 1 √ N (v1k, . . . , vNk) T , k = 1, . . . ,K, where {vik, i, k = 1, . . .} are independent and identically distributed random variables with Ev11 = 0 and Ev 2 11 = 1. Let Sk = (s1, . . . , sk−1, sk+1, . . . , sK), Pk = diag(p1, . . . , pk−1, pk+1, . . . , pK) and βk = pks T k (SkPkS T k + σ 2 I)sk, where pk ≥ 0 and the βk is referred to as the signal-to-interference ratio (SIR) of user...

Three chromosomal factors called Spore killer (Sk) have been found in wild populations of Neurospora sitophila and N. intermedia. Sk resembles other examples of meiotic drive such as Segregation Distorter in Drosophila, Pollen killer in wheat, and Gamete eliminator in tomato. In crosses heterozygous for Sk, each ascus contains four viable black ascospores and four inviable, undersize, clear asc...

Circulating endothelial progenitor cells (EPCs) are incorporated into foci of neovascularization where they undergo differentiation to mature endothelial cells (ECs). We show here that the enzyme sphingosine kinase-1 (SK-1) regulates the rate and direction of EPC differentiation without effect on the hematopoietic compartment. EPCs have high levels of SK-1 activity, which diminishes with differ...

Brucella abortus proteins from virulent S2308 expressed from a pBluescript II SK- genomic library stimulated peripheral blood mononuclear (PBM) cell proliferation from cattle vaccinated with B. abortus S19. The method described here permits a rapid and directed approach to isolate genes encoding antigens of B. abortus that interact with lymphocytes primed to the living bacterium. The supernatan...

OBJECTIVE A key mediator of vascular EC barrier integrity, S1P, is derived from phosphorylation of sphingosine by the SK-1 and SK-2. While previous work indicates that SK-1 can regulate EC barrier integrity, whether SK-2 has a similar role remains to be determined. METHODS A cell impedance assay was used to assess human umbilical vein EC and bone marrow EC barrier integrity in vitro, with app...

Human granulocyte colony stimulating factor (hGCSF) induces proliferation and differentiation of granulocyte progenitor cells. This glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. So far, different researchers have tried to enhance hGCSF biological activity and stability. In this study, Polymerase Chain Reaction (PCR...

In this paper, we are interested in the moments of the characteristic polynomial Zn(x) of the n× n permutation matrices with respect to the uniform measure. We use a combinatorial argument to write down the generating function of E [ ∏p k=1 Z sk n (xk) ] for sk ∈ N. We show with this generating function that limn→∞ E [ ∏p k=1 Z sk n (xk) ] exists for maxk |xk| < 1 and calculate the growth rate ...