We compared the modes of action of human pancreatic, human salivary, and porcine pancreatic amylases on Cibachron Blue F3GA-amylose. Both human enzymes showed similar catalytic activity with almost equal Vmax but dissimilar apparent Km's. The ratios of soluble dyed oligosaccharides to reducing substances were identical. Porcine pancreatic amylase exhibited less than half the Vmax of the human e...

Digestive health is critically dependent on the secretion of enzymes from the exocrine pancreas to the duodenum via the pancreatic duct. Specifically, pancreatic trypsin is a major protease responsible for breaking down proteins for absorption in the small intestine. Gelatin-based hydrogels, deposited in the form of thin films, have been studied as potential sensor substrates that hydrolyze in ...

INTRODUCTION High prevalence of elevated serum pancreatic enzymes in children with cholestasis with normal fecal elastase-1 concentrations has been documented. However, this state is related predominantly to biliary atresia. Therefore, we aimed to assess pancreatic damage by measuring serum pancreatic enzymes in patients with progressive familial intrahepatic cholestasis type 2 (PFIC type 2). ...

We have studied the secretin-pancreozymin stimulation test of pancreatic function with particular regard to its usefulness in the diagnosis of cystic fibrosis patients without gastrointestinal symptoms. We have compared the test results in 38 control individuals, 14 patients with cystic fibrosis and five other patients with problems affecting pancreatic function. Of the cystic fibrosis patients...

In exocrine pancreatic insufficiency (EPI) there is insufficient production or secretion of digestive enzymes by the pancreas with associated maldigestion. Usually multiple enzymes need to be affected to show clinical disease, although there is one report of a patient with a lipase-only defect. Since lipase is the rate-limiting enzyme in pancreatic digestion, deficiency in just that single enzy...

Twenty healthy subjects have been selected for the present study. There was significant parallelism between trypsin and lipase secretion but frequent dissociation between trypsin and amylase. Lower values of pancreatic volume, bicarbonate and enzymes secretion as compared to the Western reports are explained by the lower weight of Indians in comparison to Western people. Prostigmine an inexpens...

The likeliest explanation for these fatty lesions is that they are produced by pancreatic enzymes which have entered the circulation either directly or via lymphatics. The latter is thought to be the more important route. Abdominal peritoneal lymphatic drainage and transdiaphragmatic lymphatic absorption of abdominal fluid are probably prime factors in the ingress of pancreatic enzymes to the t...

Historically, large doses of proteolytic enzymes, along with diet, nutritional supplements, and "detoxification" procedures, have been used in alternative therapies to treat all forms of cancer, without formal clinical studies to support their use. A 2-year, unblinded, 1-treatment arm, 10-patient, pilot prospective case study was used to assess survival in patients suffering inoperable stage II...

CONTEXT Obstructive jaundice in chronic pancreatitis is generally caused by stenosis of the bile duct in the pancreas. Obstructive jaundice caused by the impaction of a pancreatic stone in the papilla is markedly rare, with only seven cases reported to date. CASE REPORT We report a case of obstructive jaundice caused by the impaction of a pancreatic stone in the papilla. A 41-year-old male wi...

EDITORIAL SYNOPSIS Trypsin and chymotrypsin activity per gram of stool varies inversely with the weight of the sample in control African subjects. The total daily output of both enzymes in patients with pancreatic calcification does not differ significantly from the output of control subjects. The lower mean concentration of the enzymes in chronic pancreatic disease can be accounted for by the ...