نتایج جستجو برای: page protein analysis
تعداد نتایج: 3877308 فیلتر نتایج به سال:
Blue native polyacryamide gel electrophoresis is a special case of native electrophoresis for high resolution separation of enzymatically active protein complexes from tissue homogenates and cell fractions. The method is powerful between 10 and 10,000 kDa. Also membrane protein complexes are separated well after solubilization of complexes with mild neutral detergents. The separation principle ...
Background and Aim: Antioxidants are compounds that protect cells from attacks of free radicals. Lack of balance between antioxidants and free radicals results in oxidative stress, which ultimately results in cell damage. The effects of flavonoids are not related solely to their antioxidant properties, but also to their effects on cellular signal pathways. The present study was conducted to eva...
background: infectious bursal disease virus (ibdv) causes a highly immunosuppressive disease in chickens and is a pathogen of major economic importance to the poultry industry worldwide. the vp2 protein is the major host-protective immunogen of ibdv and has been considered as a potential subunit vaccine against the disease. vp2 coding sequence was cloned in an inducible fungal vector and the pr...
crustacean hemocyanins represent a unique case of molecular heterogeneity among oxygen-carrying proteins. two populations of gammarus lacustris and six populations of gammarus komareki were identified and isolated from aquatic habitats of ardabil (northwest of iran). the populations were nominated based on name of isolated region. principal component analysis of morphological absolute attribute...
background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...
In the present investigation, protein extraction protocol for biofilm forming strain of P. aeruginosa has been optimized. It was observed that conventional TCA/Acetone precipitation method was insufficient for 2D PAGE analysis. So, a slight modification in protein extraction protocol has been made to make it suitable for 2D PAGE analysis. This protocol appears to be suitable for 2D PAGE analysi...
Background and Objective: Rh (Rhesus) is a highly complex blood group system in man which plays an important role in transfusion medicine. The aim of this study was the isolation of RhD protein from the membrane of RBCs. Materials and Methods: In this experimental study immunoprecipitation method with human anti-RhD polyclonal antibody was utilized for the isolation of RhD antigen from Rh+ huma...
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