Objectives were to compare circulating progesterone (P4) profile and pregnancies per AI (P/AI) of two commercial intravaginal P4 devices (PRID-Delta(®) vs CIDR(®)). In Experiment 1, ovariectomized dairy cows (PRID-Delta, n=6 vs CIDR, n=6) were sampled throughout 7 days to measure circulating P4. In Experiment 2 (PRID-Delta, n=399 vs CIDR, n=375), cows were assigned to treatments, as follows: D0...

Herein we report a rapid and sensitive surface Plasmon resonance (SPR) biosensor to detect progesterone (P4), a model analyte of small molecules. A terthiophene scaffold with an oligoethylene glycol (OEG) linker is used as an anchor for the P4-ovalbumin (OVA) immobilization on gold to form a stable, regenerable SPR surface. Subsequently, inhibition SPR immunoassays based on this terthiophene/OV...

A P4 is a chordless path of four vertices. A graph is P4-indifference if it admits an ordering < on its vertex set such that every P4 abcd has a < b < c < d or d < c < b < a. Such an ordering is called a P4-indifference ordering. The P4-indifference graphs were introduced in [Chv84] as a particular class of perfectly orderable graphs. A graph is perfectly orderable if there exists an ordering o...

Previous reports of adrenal progesterone (P4) contributions during late gestation in cattle, and ACTH-induced P4 responses in the non-pregnant heifer, prompted a retrospective investigation to evaluate the plasma P4 response and the relative ratio of plasma cortisol (CT) to P4 following ACTH administration during mid-gestation in pregnant Brahman heifers. Twenty-three pregnant (139.0 +/- 5.0 da...

Our objective was to develop treatments applied to cattle of unknown pregnancy status that would resynchronize the repeat estrus of nonpregnant females. In Exp. 1, previously inseminated dairy and beef heifers were assigned randomly to each of three treatments 13 d after AI: 1) no treatment (controls; n = 44); 2) 0.5 mg of estradiol cypionate (ECP) i.m. on d 13 and 20 at the time of insertion a...

In this paper we discuss the use of cluster based computing as a basis for portable, heterogeneous parallel computing. We describe p4, a cluster based communications library which originates from Argonne National Laboratory, and our implementation of p4 on the transputer architecture. We present an extension to p4, \lightweight p4", which we designed for use in our research into automatic progr...

The packaging of genomic RNA in members of the Cystoviridae is performed by P4, a hexameric protein with NTPase activity. Across family members such as Phi6, Phi8 and Phi13, the P4 proteins show low levels of sequence identity, but presumably have similar atomic structures. Initial structure-determination efforts for P4 from Phi6 and Phi8 were hampered by difficulties in obtaining crystals that...

This article focuses on bacteriophage P4 as a potential peptide display phage by exploring the possibility of using the P4 capsid decoration component, Psu, as a peptide carrier protein. Psu is non-essential for P4 growth but it enhances the stability of the P4 capsid by binding to its exterior. We have constructed a unique SacI cloning site in the beginning of the psu gene. This site changes t...

Previously, we demonstrated that progesterone (P4) at physiologic levels (5-500 nM) inhibited proliferation in cultured rat aortic smooth muscle cells (RASMCs) through a P4 receptor (PR)-dependent pathway. We also showed that P4-induced cell cycle arrest in RASMCs occurs when the cyclin-CDK2 system is inhibited just as p21cip1 and p27kip1 protein levels are augmented. In the present study, we f...

Previously, we showed that progesterone (P4) inhibits proliferation and migration of rat aortic smooth muscle cells (RASMCs). The P4-induced migration inhibition in RASMCs resulted from suppression of the Ras homolog gene family, member A (RhoA) activity mediated by cSrc activation. We also observed that P4 increased the formation of p27-RhoA complex in RASMCs. The aim of this study was to furt...