p16 is a negative regulator of cell proliferation and is considered a tumor suppressor protein. Alterations in p16 protein expression are associated with tumor development and progression. However, the p16 expression status in the peritumoral stroma has not been investigated in the endometrium. Therefore, we evaluated stromal p16 expression in different types of endometrial lesions using immuno...

PURPOSE Biliary tract cancer is an uncommon malignancy with a poor survival rate. We evaluated p16 gene alteration as a prognostic marker for this disease. EXPERIMENTAL DESIGN We studied p16 gene alterations by sequencing, methylation, and loss of heterozygosity of chromosome 9p in 118 biliary tract carcinomas, including 68 gallbladder cancers, 33 extrahepatic bile duct cancers, and 17 ampull...

Background—Inactivation of the tumour suppressor gene p16 (CDKN2/MTS-1/ INK4A) and K-ras mutations are among the most frequent genetic alterations in human malignancies. Aims—To investigate the tumour suppressor gene p16 and its possible association with K-ras mutations in intrahepatic cholangiocarcinomas of the liver. Methods—The status of p16 was evaluated in 41 cholangiocarcinomas by methyla...

Loss of p16(INK4A) (p16) expression is frequently associated with the development of epithelial and lymphoid malignancies. However, the frequency and significance of p16 abnormalities in multiple myeloma (MM) and the more aggressive phase of plasma cell leukemia (PCL) have not been well defined. Accordingly, the goal of this study was to define the expression and function of p16 in fresh sample...

background: the different growth mechanism and biologic behavior of the odontogenic keratocyst(okc) compared to other odontogenic cysts might be related to the proliferating capacity of itsepithelium. in this study, the aim was to evaluate and compare the distribution and staining intensityof p16 and cyclin d1 in okc and unicystic ameloblastoma (ua). materials and methods: in this descriptive a...

Monitoring cancer and aging in vivo remains experimentally challenging. Here, we describe a luciferase knockin mouse (p16(LUC)), which faithfully reports expression of p16(INK4a), a tumor suppressor and aging biomarker. Lifelong assessment of luminescence in p16(+/LUC) mice revealed an exponential increase with aging, which was highly variable in a cohort of contemporaneously housed, syngeneic ...

Expression of p16(INK4a) is elevated during ageing and replicative senescence. Here, we report the presence of an instability determinant within the 3'-untranslated region (UTR) of the p16 messenger RNA in WI-38 human diploid fibroblasts. The p16 3'UTR was found to be a specific target of AUF1, an RNA-binding protein implicated in promoting mRNA decay. Both AUF1 levels and AUF1-p16 mRNA associa...

to a variety of cellular proteins such as NF-␬B and I␬B (Hirai et al., 1994; Suzuki et al., 1996). Figure 1 shows the primary sequence of p16, along with that of other proteins in the p16 family: p15, p18, and p19, both human and murine. P16 is comprised mainly of four ankyrin repeats, a structural motif about Campus Chemical Instrument Center 33 amino acids long present in a variety of protein...

Studies have suggested that p16(ink4a) may be a surrogate biomarker for the diagnosis of cervical cancer; however, the function of p16(ink4a) in human cervical cancer cells remains largely unknown. Therefore, in this study, we aimed to investigate the role of p16(ink4a) in human cervical cancer cells. Immunocytochemistry was used to examine invasive squamous cell carcinoma and its precancerous ...

There is increasing evidence for a role of somatic cellular senescence in physiological aging but also in injury and disease. Cell cycle inhibitor p16 is the key mediator for stress and aberrant signaling induced senescence. Here we report that elevated blood pressure markedly induced p16 expression in rat kidneys and hearts, as well as in human kidneys. In kidneys from deoxycorticosterone acet...