It was found that wild type yeast Pichia pastoris can tolerate vanadate concentration as high as 25 mM in the growth medium. Moreover, four vanadate-resistant P. pastoris strains designated JC100/1, JC100/3, JC100/9 and JC100/15 exhibiting tolerance up to 150 mM vanadate were selected. Growth of P. pastoris was correlated with vanadate to vanadyl reduction and its accumulation in the growth med...

BACKGROUND Although Pichia pastoris has been successfully used to produce various recombinant heterologous proteins, the efficiency varies. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as an example to study the effect of protein amino acid sequence on secretion from P. pastoris. RESULTS The results indicated that the protein N-terminal sequence, the endo...

BACKGROUND AND OBJECTIVE The outer membrane protein W (ompW) of Vibrio cholerae is involved in stimulating the immune response via induction of protective immunity. It also plays an important role in bacterial pathogenesis by increasing the adaptability of pathogenic strains. In this study we aimed to clone V. cholerae ompW gene in the strain X-33 of Pichia pastoris. MATERIALS AND METHODS A g...

Recombinant chimeric α-amylase (Ba-Gt-amy) has been produced extracellularly in Pichia pastoris under AOX promoter. Clones of P. pastoris with multiple gene copies have been generated by multiple transformations and post-transformational vector amplification, which led to 10.7-fold enhancement in α-amylase titre as compared to a clone with a copy of the gene. The recombinant P. pastoris integra...

BACKGROUND Recombinant protein production in the methylotrophic yeast Pichia pastoris largely relies on integrative vectors. Although the stability of integrated expression cassettes is well appreciated for most applications, the availability of reliable episomal vectors for this host would represent a useful tool to expedite cloning and high-throughput screening, ameliorating also the relative...

background and objective: the outer membrane protein w ( ompw ) of vibrio cholerae is involved in stimulating the im- mune response via induction of protective immunity. it also plays an important role in bacterial pathogenesis by increasing the adaptability of pathogenic strains. in this study we aimed to clone v. cholerae ompw gene in the strain x-33 of pichia pastoris. materials and methods:...

White-rot fungus P. chrysosporium has been reported to produce complex hemicelluloses degradation enzymes including β-xylosidases. Genes encode for these enzymes have been predicted, namely β-xylosidase A and β-xylosidase B, however, the functional of these genes have not been characterized. This study aimed to clone a putative cDNA encoding for β-xylosidase B (PcXylB) from P. chrysosporium BKM...

Introduction: The development of an effective vaccine against Hepatitis C virus (HCV) is still a target of intensive vaccine research. The HCV envelope proteins E1 and E2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. Different types of expression systems have been used to express these glycoproteins. In this study, an expression system using Pichia p...

introduction: the development of an effective vaccine against hepatitis c virus (hcv) is still a target of intensive vaccine research. the hcv envelope proteins e1 and e2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. different types of expression systems have been used to express these glycoproteins. in this study, an expression system using pichia p...

Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...