1. When chick-embryo cells were treated with ultraviolet-inactivated influenza virus (Melbourne strain), interferon was produced after a lag period of about 10hr. 2. The addition of small amounts of either puromycin or p-fluorophenylalanine immediately after the virus inhibited the subsequent production of interferon. Both inhibitors primarily affected protein synthesis, and it is concluded tha...

l-2,5-Dihydrophenylalanine is extensively incorporated as a phenylalanine analogue into cell proteins. Phenylalanine-requiring Escherichia coli ATCC 9723f and sarcoma 180 grow at normal rates initially with the analogue and maximally replace 65 and 33%, respectively, of phenylalanine in the peptide residues of their cell protein without death. With the analogue alone growth of E. coli becomes n...

The effects of chloramphenicol and p-fluorophenylalanine (p-FPA) on growth, proportion of motile cells, average rate of motility, and the chemotactic response of a methionine auxotroph of Escherichia coli K-12 were studied. Kinetic studies revealed that the inhibition of chemotaxis by p-FPA can be explained by the effect on growth, proportion of motile cells, and average rate of motility rather...

The substantia nigra is among the richest pro-dynorphin terminal field regions in the rat brain. We therefore contrasted processing in this area to the known processing in the posterior pituitary. Fractionation of acid extracts of the posterior pituitary by gel filtration followed by analysis by radioimmunoassay indicated that the molar ratio of dynorphin A(l-17) to dynorphin A(l-8) averaged 1:...

We previously showed that chronic administration for 35 days of L-3,4dihydroxyphenylalanine (L-DOPA) or BL-1023 [an L-DOPA-gamma-aminobutyric acid (GABA) conjoined compound] improved locomotor function and increased numbers of tyrosine hydroxylase (TH) immunoreactive (TH+) neurons in the substantia nigra of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice compared to those given...

Grape (Vitis vinifera L. var. Albariño) and mulberry (Morus nigra L.) seeds pomace were characterized in terms of tocopherols, organic acids, phenolic compounds bioactive properties. Higher contents tocopherols (28 ± 1 mg/100 g fw) obtained mulberry, whilst grape richer acids (79 4 fw). The analysis hydroethanolic extracts characterised by catechin oligomers (36.0 0.3 mg/g) ellagic acid derivat...

increasing of wood demand is currently one of the most important problems in our society. different clones of populus nigra from salicacea as a native fast-growing species have a good potential for planting in wide scale. but because of the ecological differences in the field, especially. temperature alterations, segregation of the cold resistant clones in relation to in seedling stage establis...

Background and aim: Diabetic nephropathy results from an increasing in oxidative stress. The phenolic components of mulberry (Morus nigra L.) leaves extract have antioxidant components and may modulate oxidative stress. In this study, the beneficial effects of mulberry leaves extract were assessed in diabetic nephropathy. Materials and methods: Diabetes induced by high-fat diet and injection of...

Climate change has strongly increased the fire frequency in Mediterranean forests causing changes soil bacterial, fungal and microarthropod communities. Fire impacts on properties depend vegetation covers. In this framework, aim of research was to evaluate effects communities community soils under trees shrubs a area. Surface cores were sampled unburnt burnt (three years since occurrence) patch...

embryo culture were used to produce inter specific walnut hybrid plants between juglans nigra l. and j. regia l. developed ovaries were collected from j. rejia female flowers which were pollinated with j. nigra pollen grain by high dusting on a mature tree. in order to isolate embryos, exocarps were aseptically removed. isolated embryos from ovules at different developmental stages were transfe...