Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and...

Among 1,962 bacterial isolates from a smear-surface soft cheese (Munster cheese) screened for activity against Listeria monocytogenes, six produced antilisterial compounds other than organic acids. The bacterial strain WHE 92, which displayed the strongest antilisterial effect, was identified at the DNA level as Lactobacillus plantarum. The proteinaceous nature, narrow inhibitory spectrum, and ...

The microbiology of Kasseri cheese, a Protected Designation Origin (PDO) cheese pasta-filata type was studied in order to identify the dominant species and strains that may contribute maturation process. Chemical composition microbiological quality samples from two different dairies during at 0, 7, 25, 60 90 days maturation. Lactic acid bacteria Enterococcus spp. were found be microflora fresh ...

A total of 83 acid-producing strains were isolated from fresh Lentinus edodes, Pleurotus eryngii, Flammulina velutipes, Agaricus bisporus, ostreatus, djamor, abalones, and citrinopileatus by CaCO3-MRS plate medium. These divided into 9 species, including 52 Lactococcus lactis, 13 Pediococcus pentosaceus, 8 Enterococcus faecium, 3 garvieae, 2 casseliflavus, Lactobacillus plantarum, 1 strain acid...

Microbial activity is an integral part of agricultural ecosystems and can influence the quality food commodities. During on-farm processing, coffee growers use a traditional method fermentation to remove cherry pulp surrounding beans. Here, we investigated farm microbiome resulting process conducted with selected starter cultures (Pichia fermentans YC5.2 Pediococcus acidilactici LPBC161). The m...

The probiotics are being used as ecofriendly and bioremediation tools for developing sustainability to aquaculture. present study was conducted explore the practical capability of using dietary lactic acid bacteria (Pediococcus acidilactici) see how its dose variation affected water quality, growth performance, survival rate, body composition, blood biochemical parameters, intestinal histology ...

Indonesia has many kind of fermented foods such as tempe, fermented rice, fermented cassava, and fermented vegetable. Lactic acid bacteria that can produce β glycosidase most likely in the fermented foods from plants materials. The objectives of the research were to isolate lactic acid bacteria producing β glucosidase enzyme from Indonesian fermented foods and identify the isolates to their spe...

Coccidiosis is the major parasitic disease of poultry. In this study, the role of the commercial probiotic MitoMax which contains Pediococcus acidilactici and Saccharomyces boulardii was evaluated by measuring body weight gain, fecal oocyst shedding, and serum antibody responses as an alternative control method of prophylactic drug against coccidiosis. Day-old broiler chicks were fed regular or...