Abstract To discover new drugs to combat COVID-19, an understanding of the molecular basis SARS-CoV-2 infection is urgently needed. Here, for first time, we report crucial role cathepsin L (CTSL) in patients with COVID-19. The circulating level CTSL was elevated after and positively correlated disease course severity. Correspondingly, pseudovirus increased expression human cells vitro ACE2 tran...

Background: MicroRNAs (miRNAs) are noncoding RNAs that control the expression of their target mRNAs. It affects cancer cell proliferation and apoptosis as oncogenes or tumor suppressors. Dysregulation of miRNAs expression leads to the development of various cancers. Therefore, for the first time in this field, this study investigated the effect of overexpression of microRNA-630 on the Jurkat ce...

Objective(s): The prognosis of osteoporosis is very poor, and it is very important to identify a biomarker for prevention of osteoporosis. In this study, we aimed to identify candidate markers in osteoporosis and to investigate the role of candidate markers in osteogenic differentiation. Materials and Methods: Using Weighted Gene Co-Expression Network analysis, we identified three hub genes mig...

We investigated the effect of functional point mutations (Q730E) endoplasmic reticulum aminopeptidase 1 (ERAP1) on proliferation, apoptosis and cytokine secretion HaCaT cells. The ERAP1 ERAP1-Q730E were overexpressed in expression TNFR1 TNFR2 cells was detected by immunofluorescence. proliferation CCK-8. Cell cycle analyzed flow cytometry. IL-1β, TNF-α IL-6 ELISA. IKKs caspase-8 Western blot. o...

Overexpression of asip1 in transgenic zebrafish disrupts dorsoventral pigment pattern addition to increasing food intake levels and linear growth. A higher feed is unnecessary fish enable larger heavier plausible explanation may rely on the enhanced feeding efficiency mediated by improved nutrient absorption animals. To test this hypothesis, wide scope transcriptomic techniques were used elucid...

Juvenile hormone (JH) plays a vital role in the growth, development, and reproduction of insects other arthropods. Previous experiments have suggested that BmFAMeT6 could affect duration silk moth’s larval stage. In this study, we established overexpression strain knockout using GAL4/UAS binary hybrid system CRISPR/Cas 9 system, respectively, found stage was shorter, while longer. Our results e...

The hematopoietic system plays a crucial role in immune defense response and normal development, it is regulated by various factors from other tissues. dysregulation of hematopoiesis associated with melanotic mass formation; however, the molecular mechanisms underlying this process are poorly understood. Here, we observed that overexpression miR-274 fat body resulted formation masses. Moreover,...

Background: As the third most frequent cause of cancer death, breast cancer is a common disease worldwide. Most of the patients are being diagnosed in the stage that conventional treatments are not effective, and invasion and metastases lead to death. Therefore, identification of novel molecular markers to improve early diagnosis, prognosis and treatment of the breast cancer is a necessity. Zin...