Fas ligand (FasL) and its receptor Fas have been implicated in granulosa cell apoptosis during follicular atresia. Although interferon-gamma (IFN-γ) is believed to be involved in the regulation Fas expression in differentiated granulosa or granulosa-luteal cells, the expression of this cytokine and its role in the regulation of the granulosa cell Fas/FasL system and apoptosis during follicular ...

A-kinase anchoring proteins (AKAPs) function to target protein kinase A (PKA) to specific locations within the cell. AKAPs are functionally identified by their ability to bind the type II regulatory subunits (RII) of PKA in an in vitro overlay assay. We previously showed that follicle-stimulating hormone (FSH) induces the expression of an 80-kDa AKAP (AKAP 80) in ovarian granulosa cells as they...

Background: Ovarian cancer is common amongst women worldwide. In Indonesia, it the fourth most female with a 5-year survival rate of 45%, but can reach up to 92% if treated early. Granulosa cell tumors (GCT) arise from sex-cord and are considered malignant. This study presents rare case asymptomatic adult-type granulosa tumor in an elderly lady diagnosed during routine pelvic ultrasound scannin...

Ovarian follicular fluid peptide (OFFP) purified from sheep ovaries enhances apoptotic changes in ovarian granulosa cells of mice. To get an insight into the cell subpopulations responding to OFFP, the heterogeneity of granulosa cells was resolved. Subpopulations of granulosa cells were obtained from ovaries of immature mice treated with PMSG alone and autopsied 48 hr (control) and 72 hr after ...

Orphan nuclear receptors such as germ cell nuclear factor (GCNF), steroidogenic factor 1 (SF-1) and liver receptor homolog-1 (LRH-1), are emerging as important ovarian factors in regulating female reproduction. Within the ovary, GCNF (NR6A1) expression is restricted to the oocyte, while SF-1 (NR5A1) is expressed only in the somatic cells, such as granulosa, thecal and luteal cells, and intersti...

The production of ovarian neoplaslns in female mice by x-rays was described in 1936 (7, 10). q]ae tmnors arose about 7 to 10 months following irradiation with single or multiple doses of 200 r to 400 r. These tumors were of 3 histological types: (a) granulosa cell tumors, (b) luteomas, and (c) tubular adenomas. Whether the gland or duct-like structures of some of these adenomas are related to a...

The direct effect of insulin and insulin-like growth factor-Ion ovarian granulosa cell function in cattle was evaluated by using a serum-free culture system. Granulosa cells were obtained from small (1 to 5 mm) follicles collected from cattle and cultured for 4 days. Culture medium was collected for quantification of progesterone, and cell numbers were determined. Both insulin and IGF-I signifi...

We examined numerous ovarian follicles from 32-35 d virgin mice by transmission electron microscopy and light microscopic immunohistochemistry. No macrophages were seen, but various stages of apoptotic granulosa cells were encountered. Presumably a granulosa cell or its debris in an advanced stage of apoptosis was destined to be phagocytosed by adjacent normal-looking granulosa cells. Other gra...

Kalfa et al.: Genome Medicine 2009, 1:81 Abstract Granulosa cell tumors (GCTs) of the ovary belong to the group of ovarian sex-cord stromal tumors and represent 5 to 10% of ovarian malignancies. GCTs exhibit several morphological, biochemical and hormonal features of normal proliferating preovulatory granulosa cells, such as estrogen biosynthesis. Prognostic factors of this condition are lackin...

Mammalian ovarian follicular development and atresia is closely regulated by the cross talk of cell death and cell survival signals, which include endocrine hormones (gonadotropins) and intra-ovarian regulators (gonadal steroids, cytokines and growth factors). The fate of the follicle is dependent on a delicate balance in the expression and actions of factors promoting follicular cell prolifera...