OBJECTIVE Umbilical mesenchymal stem cells (UMSCs) is one of most popular regenerative medical source of bone replacement therapy in both clinical and scientific researches. However, it is still low effective to induce the osteogenesis of hUMSCs. In this study, we aimed to elucidate the roles of DNA methyltransferase 3B (DNMT3B) in the osteogenesis of hUMSCs. MATERIALS AND METHODS Knockdown D...

Osteogenesis imperfecta is a genetic disorder of type I collagen. Although multiple genotypes and phenotypes are associated with osteogenesis imperfecta, approximately 90% of the mutations are in the COL1A1 and COL1A2 genes. Osteogenesis imperfecta is characterized by bone fragility. Patients typically have multiple fractures or limb deformity; however, the spine can also be affected. Spinal ma...

Learning Objectives: After studying this article, the participant should be able to: 1. Review the biomechanical principles and pertinent cellular and molecular biology of distraction osteogenesis of the craniofacial skeleton. 2. Describe the clinical indications and applications of distraction osteogenesis of the craniofacial skeleton. 3. Describe maxillary, mandibular, midface, and calvarial ...

Distraction osteogenesis is a powerful technique for creating new bone during significant lengthening of the mandible without the need for bone grafting and associated donor site morbidity. The majority of clinical applications of mandibular lengthening with distraction osteogenesis techniques reported in the literature are in growing patients.1-8 For the purposes of this article, an adult pati...

statement of problem: it is not known whether endochondral and intramembranous bones have distinct biological characteristics. octacalcium phosphate (ocp), a hydroxyapatite precursor, has been reported to stimulate bone formation after being implanted in parietal bone defects of rats. purpose: the present study was designed to investigate the response of endochondral and intramembranous bones t...

The benefits of physical loading to skeletal mass are well known. primary cilium has emerged as an important organelle in bone mechanobiology/mechanotransduction, particularly mesenchymal stem/stromal cells, yet the molecular mechanisms mechanotransduction poorly understood. In this study, we demonstrate that Gpr161 is a mechanoresponsive GPCR, localises cilium, and involved fluid shear-induced...

Osteogenesis imperfecta is a disease characterized by increased fragility of the bones which are easily fractured by slight trauma. Patients suffering from this disorder usually have blue sclerae and flaccid ligaments; some of them become deaf later in life. Scattered reports of this disorder date back at least to 1678 (quoted by Seedorff in 1949). Vrolik (1849) described the disease in the new...

This paper evaluates changes in electricity generation costs caused by the introduction of market mechanisms to determine production United States. I use staggered transition markets from 1999 2012 estimate causal impact liberalization using a differences-in-difference design on comprehensive hourly panel demand, generators’ costs, capacities, and output. find that reduce 5 percent reallocating...

objectives: the purpose of this study was to evaluate whether intra-canal application of controlled direct electric current (dec) could accelerate the amount of orthodontic tooth movement (otm) in young adult mixed breed dogs. methods: fourth premolars of both jaws of five young adult mixed breed dogs were divided into two groups of control and experimental groups. orthodontic force was applied...

Objective(s): Naringin has been reported to regulate bone metabolism. However, its effect on osteogenesis remains unclear. The aim was to investigate the effect of naringin on osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) through the activation of the ERK signaling pathway in osteogenic differentiation. Materials and Methods: Annexin V-FITC assay and MTT assay ...